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TWIN Ecosystems Project


EMSL Project ID
60272

Abstract

The goal of the TWIN ecosystem project (‘TWINS’) is to pilot laboratory and field ‘twin’ ecosystems that use sensors and autonomous controls to test the hypothesis that compositional changes in root exudates during drought stress select for beneficial rhizosphere microbes. To do this, TWINS brings together unique resources in fabricated ecosystems at Berkeley Lab, field ecology expertise and resources at PNNL, sensor and omics expertise at EMSL, lab automation and omics expertise at JGI, and the mathematical and computational expertise of CAMERA. TWINS builds on existing resources for investigating molecular interactions in the rhizosphere to gain insights into whether tall wheatgrass exudates select rhizosphere communities in response to drought, enabling us to leverage an existing PNNL drought study using tall wheatgrass (Thinopyrum ponticum). Tall wheatgrass is a widely distributed species adapted to dry northern latitudes that is being considered as a bioenergy feedstock on marginal lands. It is known to develop soil “resource islands” or “hot spots” that may impose heterogeneous spatial distribution of important plant exudates impacting the soil microbiome, especially in response to drought when plants may differentially allocate photosynthates to particular roots. The field ‘twin’ will define climate conditions and hyperspectral signatures of drought stress enabling the lab ‘twin’ to characterize the composition, localization, and dynamics of microbes and exudates—providing powerful environmental controls and measurements which are essentially not possible in the field.

Project Details

Start Date
2021-12-06
End Date
N/A
Status
Active

Team

Principal Investigator

Nancy Hess
Institution
Environmental Molecular Sciences Laboratory

Co-Investigator(s)

James Moran
Institution
Michigan State University

Kirsten Hofmockel
Institution
Pacific Northwest National Laboratory

Susannah Tringe
Institution
Lawrence Berkeley National Laboratory

Trent Northen
Institution
Lawrence Berkeley National Laboratory

Team Members

Gregory Vandergrift
Institution
Pacific Northwest National Laboratory