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Structural profiling of the molecular interactome to define phenotype


EMSL Project ID
60459

Abstract

The functional pathways that define phenotype are reflected by protein quinary structure; the transient encounters of proteins with other proteins, molecules, cofactors, and nucleic acids that are responsible for the molecular organization of a biological system. This project will develop and integrate three complementary technologies that leverage liquid chromatography-mass spectrometry (LC-MS) to measure protein quinary structure: (i) thermal proteome profiling (TPP), (ii) limited proteolysis (LiP), and (iii) fast photochemical oxidation of proteins (FPOP). Together, these techniques will provide comprehensive structural and functional data required for predicting and engineering biological phenotypes, the primary mission of the Predictive Phenomics Initiative. Pursuit to this objective, we have two primary aims:

Project Details

Start Date
2022-04-05
End Date
N/A
Status
Active

Team

Principal Investigator

John Melchior
Institution
Pacific Northwest National Laboratory

Team Members

Madelyn Berger
Institution
Pacific Northwest National Laboratory

Song Feng
Institution
Pacific Northwest National Laboratory

Tong Zhang
Institution
Pacific Northwest National Laboratory

Isaac Attah
Institution
Pacific Northwest National Laboratory

Chelsea Hutchinson
Institution
Pacific Northwest National Laboratory

Weijun Qian
Institution
Pacific Northwest National Laboratory