Developing an automation-ready high throughput chemoproteomic workflow
EMSL Project ID
60473
Abstract
We propose to develop a high-throughput (HTP) workflow for activity-based protein profiling (ABPP) or bioorthogonal non-canonical amino acid tagging (BONCAT) samples. This HTP chemoproteomic sample preparation workflow will include a fluorescence readout to rapidly assess sample quality (e.g. labeling efficiency) in all samples and facilitate the transition to a 96-well format for automated liquid handling and sample processing. All steps of ABPP sample preparation, from click chemistry, enrichment, digestion, and tandem mass tag (TMT) labeling of peptides, will be optimized for 96-well format. Development of this HTP workflow will make ABPP and other chemoproteomic approaches less labor intensive (lower cost) and more accessible to EMSL users.
Project Details
Start Date
2022-10-01
End Date
N/A
Status
Active
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Team Members