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Single-Molecule and Single-Particle Spectroelectrochemistry by Super- Resolution Fluorescence Imaging


EMSL Project ID
60517

Abstract

In chemical and biological systems, interfacial redox processes play important roles. Redox reactions are ubiquitous in biological systems, from metabolism and nutrient utilization from the environment. A deeper understanding of redox reactions would address multiple unsolved problems in chemical bond formation, molecular dynamics, and redox protein chemistry. By synchronizing electrochemical potential scanning with a single-molecule localization super-resolution fluorescence microscope, kinetic fluorescence changes of hundreds of single molecular redox events were tracked simultaneously in high throughput. In this project, we will firstly study the interaction of a few typical redox dyes with nanoparticles using single-molecule methods. We will investigate whether there is any electron or energy transfer between the fluorescent redox mediators and the nanoparticles. Next, we will introduce the redox proteins (enzymes) into the system to be electrochemically oxidized and reduced via the fluorescent redox mediators, thereby exploring kinetics of interfacial electron transfer and redox reactions in biological systems. Our strategy is to crossly integrate electrochemistry and super-resolution fluorescence microscopy, create a high-level basic research platform, promote the development and fusion of branch knowledges, and produce major scientific breakthroughs through interdisciplinary research.

Project Details

Start Date
2022-06-14
End Date
N/A
Status
Active

Team

Principal Investigator

Dehong Hu
Institution
Environmental Molecular Sciences Laboratory

Team Members

Chenghong Lei
Institution
Guilin University of Technology