A 3D printed SubTap platform for high-throughput plant-microbe interaction studies
EMSL Project ID
60568
Abstract
High-throughput (HTP) mass spectrometry will provide a holistic analysis of microbial and plant community interactions. The recently developed 3D printed SubTap platform (through a collaborative work between University of Pittsburgh and EMSL) (Fig. 1)1, represents a HTP platform that provides reliable and rapid mass spectrometry analysis of microbial community interactions by capturing the exometabolome resulting from microbes grown in physically separate but interconnected growth compartments. Here, we propose to develop a “SubTap Rhizosphere platform,” called SubTapRhizo, for large scale and HTP interrogation of the exometabolome arising from plant-microbe interactions. Through multiculture in spatially mixed or unmixed configurations, the SubTapRhizo platform will allow for rapid chemical phenotyping of several sets of plant-microbe interactions at a time. Moreover, the SubTapRhizo platform will be compatible with plant growth in agar, native or synthetic soil providing users a wide range of options to analyze plant-microbe interactions.
Project Details
Start Date
2022-10-24
End Date
2023-10-01
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members