Preserving plant tissue section integrity and introducing ionic organic matrices to strengthen plant MALDI MSI capability at EMSL
EMSL Project ID
60590
Abstract
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) is the most utilized capability for spatial metabolomics in EMSL. Still, it can be significantly improved to deliver more accurate, faster, and comprehensive data to EMSL users. The first objective of this proposal is to improve plant tissue's preparation and sectioning methodology to preserve tissue integrity, which will enable accurate information on the cellular origin of molecules detected in MALDI MSI, and at a broader scale, provide better tissues for other microscopy-focused techniques. The second objective is to develop an ionic MALDI matrix application workflow to improve high throughput and expand molecular coverage from plant tissues. This will significantly advance EMSL MALDI MSI capabilities, align with rhizosphere sensors and HTP omics RAs, and target BER’s mission of understanding genotype-phenotype relationships. It will significantly impact the quality of the imaging data we deliver to EMSL users who aim to understand how plant cells spatially interact with the (micro)environment.
Project Details
Start Date
2022-11-01
End Date
2023-09-01
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members