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Super resolution fluorescence imaging of plant receptors

EMSL Project ID


This proposal covers work done under a DOE BER funded research project led by the University of Missouri. The project focuses on developing and demonstrating the application of a fluorescence microscopy platform, including the development of visible and near infrared (NIR) fluorescent quantum nanomaterials, to enable high resolution multiplexed imaging in plants. The overlapping emission spectra of endogenous plant pigments currently limit the number of fluorophores with different emission colors that can be imaged simultaneously and, therefore, the number of cellular components that can be imaged within a single experiment. The endogenous plant pigments also increase background and reduce image quality in fluorescence images. Hence, developing fluorescent tags and a microscope system that can image beyond the visible range deep into the NIR would greatly enhance plant imaging capabilities. Extending imaging into the NIR region would support the interrogation of multiple cellular molecular targets simultaneously. Efforts pursued at the University of Maryland Baltimore County will focus on the development of polymer dots (Pdots) that emit both at the visible and NIR regions. The application of the new imaging platform will be demonstrated by studying the functional identity of these membrane receptors in high spatial resolution.

Project Details

Start Date
End Date


Principal Investigator

Dehong Hu
Environmental Molecular Sciences Laboratory


Galya Orr
Environmental Molecular Sciences Laboratory

Team Members

Mowei Zhou
Environmental Molecular Sciences Laboratory

Christopher Anderton
Environmental Molecular Sciences Laboratory