Skip to main content

Determining the roles of sphingolipids in phagocytosis


EMSL Project ID
60632

Abstract

Phagocytosis is a conserved cellular process that is not only critical to ingest and eliminate invading microbial pathogens but also to clear effete cells, and cellular debris. Phagocytosis also relies on spatial-temporal modification of lipids that compose the plasma membrane. These include the signaling lipid molecules the phosphoinositides (PIs). However, the roles of other lipids including sphingolipids (SPLs), molecules enriched at the outer leaflet of the plasma membrane, in phagocytosis remain largely unknown. We hypothesize that both structural and bioactive SPLs are critical determinant factors in phagocytosis and promote the recruitment of phagosomal proteins to the phagocytic synapse. The goal of the proposal is to systematically perturb the key SPL pathways of the host to uncover their function in phagocytosis. This contribute towards goal we aim to identify determine the SPL species and other complex membrane lipids required for phagocytosis

Project Details

Start Date
2022-10-24
End Date
N/A
Status
Active

Team

Principal Investigator

Jennifer Kyle
Institution
Pacific Northwest National Laboratory

Team Members

Josie Hansen
Institution
Pacific Northwest National Laboratory

Kelly Stratton
Institution
Pacific Northwest National Laboratory