SIV/HIV Reservoir characterization utilizing nanoscale proteomics
EMSL Project ID
60652
Abstract
HIV cannot be eradicated by antiretroviral therapies (ART) alone. Although lifelong suppression of HIV replication with ART seems possible, side effects, need for strict adherence, resistance, stigma and cost all contribute to the necessity of finding an ‘HIV cure’. The major obstacle to eradicating HIV is the persistence of cellular viral reservoirs (VR) harboring replication competent viral genomes that have the capacity to produce infectious virus. Thus, the overarching goals of this research proposal are to merge innovative in situ hybridization (ISH) approaches to quantify and map VR at high resolution with multiple new cutting-edge multi-omics platforms to investigate mechanisms of HIV persistence at the single-cell level while retaining critically important contextual insight into the cellular immune neighborhoods and inflammatory landscapes in which VR reside. We will utilize advanced proteomics capabilities at PNNL (including SNaPP and nanoPOTS) to perform unbiased proteomic analysis on cells and/or tissues provided by the Estes laboratory at OHSU using mass spectrometry to characterize the landscapes in which VR resides. The Estes lab will provide PNNL with macaque cells and/or tissues from lymphoid tissues (peripheral and mesenteric lymph nodes and spleen) and gastrointestinal (GI) tract before and at different time points during ART.
Project Details
Start Date
2022-12-01
End Date
N/A
Status
Active
Released Data Link
Team
Principal Investigator