Nanodroplet sampling processing platform for amine containing metabolites in single Yarrowia lipolytical fungal protoplasts
EMSL Project ID
60842
Abstract
Single-cell metabolomics of bioproduction organisms such as Yarrowia lipolytica could significantly enhance our understanding of functional heterogeneity, yet remains a challenge due to limited input material, low retention on liquid-chromatography (LC) columns, and poor mass spectrometry (MS) ionization efficiency. To address these challenges, we propose using the cellenONE single-cell isolation system in combination with nanoPOTS and tandem mass tags (TMT) to create a state-of-the-art single-cell LC-MS metabolomics workflow. The cellenONE will enable facile isolation of these smaller (5-8 µm) single-cells while nanoPOTS will minimize surface adsorption and sample loss. To further resolve trace sample size issues, amine-containing metabolites will be reacted with TMT to improve LC retention, ionization efficiency, and boost-signal via a TMT reference channel. We aim to enhance EMSL’s metabolomics capability and extend its application to high-throughput single-cell metabolomic profiling of fungal and plant cells.
Project Details
Start Date
2023-04-17
End Date
N/A
Status
Active
Released Data Link
Team
Principal Investigator
Team Members