Surface Induced Dissociation of Polyatomic Ions (J Laskin's BES, PNNL Scope #40457)
EMSL Project ID
6291
Abstract
The general objective of our research is to achieve a fundamental understanding of the reaction kinetics and dynamics of activating and dissociating complex molecular ions. These processes are the scientific foundation for tandem mass spectrometry, one of the most important analytical methods developed in the last century. We investigate the collisional activation and dissociation of protonated model peptides, a particularly important class of complex molecules whose characterization by tandem mass spectrometry is central to the emerging field of proteomics. Our results obtained thus far indicate that collisional activation of ions in a single collision with a specially prepared surface - surface-induced dissociation (SID) - is a promising method for investigating fundamental questions of ion activation and may be a preferred method for practical applications, especially in Fourier Transform Ion Cyclotron Resonance mass spectrometry. We will investigate dynamics of the surface-induced dissociation of large molecules, the influence of physical and chemical properties of SID targets on energy transfer efficiency and neutralization of complex ions using collision energy-resolved and angular-resolved measurements. We will also apply theoretical models to extract the energetics and dynamics of complex ion dissociation processes from the experimental data.Project Details
Start Date
2003-01-01
End Date
2007-06-03
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members