Surface Induced Dissociation of Polyatomic Ions (J Laskin's BES, PNNL Scope #40457)
EMSL Project ID
6291
Abstract
The general objective of our research is to achieve a fundamental understanding of the reaction kinetics and dynamics of activating and dissociating complex molecular ions. These processes are the scientific foundation for tandem mass spectrometry, one of the most important analytical methods developed in the last century. We investigate the collisional activation and dissociation of protonated model peptides, a particularly important class of complex molecules whose characterization by tandem mass spectrometry is central to the emerging field of proteomics. Our results obtained thus far indicate that collisional activation of ions in a single collision with a specially prepared surface - surface-induced dissociation (SID) - is a promising method for investigating fundamental questions of ion activation and may be a preferred method for practical applications, especially in Fourier Transform Ion Cyclotron Resonance mass spectrometry. We will investigate dynamics of the surface-induced dissociation of large molecules, the influence of physical and chemical properties of SID targets on energy transfer efficiency and neutralization of complex ions using collision energy-resolved and angular-resolved measurements. We will also apply theoretical models to extract the energetics and dynamics of complex ion dissociation processes from the experimental data.Project Details
Start Date
2003-01-01
End Date
2007-06-03
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
J. J. Hache, J. H. Futrell and J. Laskin. “Relative Proton Affinities from Kinetic Energy Release Distributions for Dissociation of Proton-Bound Dimers: 2. Diamines as a test case.” Int. J. Mass Spectrom., Tilmann Märk Sp. Issue, 233, 223-231 (2004).
J. Laskin and J.H. Futrell. “Activation of Large Ions in FT-ICR Mass Spectrometry.” Mass Spectrom. Rev., Invited Review, 24, 135-167 (2005).
J. Laskin and J.H. Futrell. “Collisional Activation of Peptide Ions in FT-ICR Mass Spectrometry.” Mass Spectrom. Rev., Invited Review, 22, 158-181 (2003)
J. Laskin and J.H. Futrell. “Energy Transfer in Collisions of Peptide Ions with Surfaces.” J. Chem. Phys., 119, 3413-3420 (2003).
J. Laskin and J.H. Futrell “Entropy is the Major Driving Force for Fragmentation of Proteins and Protein-Ligand Complexes in the Gas-Phase.” J. Phys. Chem. A, 107, 5836-5839 (2003).
J. Laskin and J.H Futrell. "Surface-Induced Dissociation of Peptide Ions: Kinetics and Dynamics." J. Am. Soc. Mass Spectrom., 14, 1340-1347 (2003).
J. Laskin. “Energetics and Dynamics of Peptide Fragmentation from Multiple-Collision Activation and Surface-Induced Dissociation Studies.” Eur. J. Mass Spectrom., Jean Futrell and Burnaby Munson Sp. Issue, 10, 259-267 (2004).
J. Laskin. “Energy and Entropy Effects in Gas-Phase Dissociation of Peptides and Proteins,” in Principles of Mass Spectrometry Applied to Biomolecules, J. Laskin and C. Lifshitz (Eds.), Wiley, Hoboken, NJ, 2006, pp. 619-665.
J. Laskin- “Fragmentation Energetics of Protonated Leucine Enkephalin from Time-and Energy-Resolved Surface-Induced Dissociation Studies”, J. Phys. Chem. A, 110, 8554-8562 (2006)
J. Laskin, K.M. Beck, J.J. Hache, J.H. Futrell. “Surface-Induced Dissociation of Ions Produced by Matrix-Assisted Laser Desorption Ionization in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer.” Anal. Chem., 76, 351-356 (2004).
J. Laskin, T.H. Bailey and J.H. Futrell. “Fragmentation Energetics for Angiotensin II and its Analogs from Time-and Energy-Resolved Surface-Induced Dissociation Studies.” Int. J. Mass Spectrom., Alan Marshall Sp. Issue, 234, 89–99 (2004).
J. Laskin, T.H. Bailey and J.H. Futrell “Mechanisms of Peptide Fragmentation from Time-and Energy-Resolved Surface-Induced Dissociation Studies: Dissociation of Angiotensin Analogs,” Int. J. Mass Spectrom., Chava Lifshitz Memorial Issue, 249–250, 462–472 (2006)
J. Laskin, T.H. Bailey and J.H. Futrell. “Shattering of Peptide Ions on Self-Assembled Monolayer Surfaces.” J. Am. Chem. Soc., 125, 1625-1632 (2003).
T. H. Bailey, J. Laskin and J. H. Futrell. “Energetics of Selective Cleavage at Acidic Residues Studied by Time-and Energy-Resolved Surface-Induced Dissociation in FT-ICR-MS.” Int. J. Mass Spectrom., Jack Beauchamp Sp. Issue, 222, 313-327 (2003).
Z. Yang, O. Hadjar and J. Laskin. “Effect of the Surface Morphology on the Energy Transfer in Ion-Surface Collisions,” Int. J. Mass Spectrom., Jean Futrell Honor Issue, 265, 124–129 (2007).