Selective immobilization of biological molecules on single walled carbon nanotube (SWNT) surfaces
EMSL Project ID
7994
Abstract
Selective immobilization of biological molecules on single walled carbon nanotube (SWNT) surface is very interesting and important topic for various nanoscale biosensor applications. Confirmative spectroscopic investigation of selective (or non-specific) binding of biomolecules, such as proteins and DNA on the surface of SWNT as well as their stability is very critical for systematic studies. Highly pure SWNTs are synthesized by chemical vapor deposition (CVD) process in NMRL at POSTECH and the systems for the spectroscopic studies are prepared by immobilizing dye-tagged protein molecules on top of SWNT surface. Dye-Streptavidin and Q-dot-Streptavidin systm will be the main targets. The immobilization process is based on the non-specific binding property of protein molecules on the surface of SWNT. I would like to conduct a Fluorescence Lifetime Imaging (FLIM) experiment in the lab of Dr. Peter Lu. This facility had been constructed by Peter?s group members, including myself. Therefore I am already familiar with the machine and expect to produce valuable Single Molecule Fluorescence Lifetime data during my visit. Expected result will be relevant with the information of chemical interactions of protein or DNA molecule with SWNT molecule. Sample will be prepared on a very thin quartz plate or slide cover glass and pre-observed with either AFM-FLIM Microscope or Field Emission Scanning Electron Microscope.
Project Details
Project type
Exploratory Research
Start Date
2004-04-05
End Date
2005-04-18
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
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