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EMSL CAT IN ANALYTICAL MASS SPECTROMETRY


EMSL Project ID
7996

Abstract

The general objective of our research is to achieve a fundamental understanding of the reaction kinetics and dynamics of activating and dissociating complex molecular ions. These processes are the scientific foundation for tandem mass spectrometry, one of the most important analytical methods developed in the last century. We investigate the collisional activation and dissociation of protonated model peptides, a particularly important class of complex molecules whose characterization by tandem mass spectrometry is central to the emerging field of proteomics. Our results obtained thus far indicate that collisional activation of ions in a single collision with a specially prepared surface - surface-induced dissociation (SID) - is a promising method for investigating fundamental questions of ion activation and may be a preferred method for practical applications, especially in Fourier Transform Ion Cyclotron Resonance mass spectrometry. We investigate dynamics of the surface-induced dissociation of large molecules, the influence of physical and chemical properties of SID targets on energy transfer efficiency and neutralization of complex ions using collision energy-resolved and angular-resolved measurements. We also apply theoretical models to extract the energetics and dynamics of complex ion dissociation processes from the experimental data.

Project Details

Start Date
2004-03-03
End Date
2006-10-04
Status
Closed

Team

Principal Investigator

Julia Laskin
Institution
Purdue University

Team Members

Peng Wang
Institution
Pacific Northwest National Laboratory

Zhibo Yang
Institution
University of Colorado at Boulder

Jason Green
Institution
Purdue University

Omar Hadjar
Institution
O.I. Analytical

Facundo Fernandez
Institution
Georgia Institute of Technology

Partha Basu
Institution
Duquesne University

Robert Cooks
Institution
Purdue University

William Hase
Institution
Texas Tech University

Jean Futrell
Institution
Pacific Northwest National Laboratory

Vicki Wysocki
Institution
The Ohio State University

Murray Johnston
Institution
University of Delaware

Hin-koon Woo
Institution
The Scripps Research Institute

Lai-Sheng Wang
Institution
Brown University

Related Publications

Alvarez J, JH Futrell, and J Laskin. 2006. "Soft-Landing of Peptides onto Self-Assembled Monolayer Surfaces." Journal of Physical Chemistry A 110(4):1678-1687.
Alvarez J, RG Cooks, SE Barlow, DJ Gaspar, JH Futrell, and J Laskin. 2005. "Preparation and in situ Characterization of Surfaces Using Soft-Landing in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer." Analytical Chemistry 77(11):3452-3460.
Fernandez FM, VH Wysocki, JH Futrell, and J Laskin. 2006. "Protein Identification Via Surface-Induced Dissociation in an FT-ICR Mass Spectrometer and a Patchwork Sequencing Approach." Journal of the American Society for Mass Spectrometry 17(5):700-709.
Fu Y, J Laskin, and LS Wang. 2006. "Collision Induced Dissociation of [4Fe-4S] Cubane Cluster Complexes: [Fe4S4C14-x(SC2H5)x]2-/1- (x=0-4)." International Journal of Mass Spectrometry 255-256:102-110.
J. J. Hache, J. H. Futrell and J. Laskin. “Relative Proton Affinities from Kinetic Energy Release Distributions for Dissociation of Proton-Bound Dimers: 2. Diamines as a test case.” Int. J. Mass Spectrom., Tilmann Märk Sp. Issue, 233, 223-231 (2004).
J. Laskin and J.H. Futrell. “Activation of Large Ions in FT-ICR Mass Spectrometry.” Mass Spectrom. Rev., Invited Review, 24, 135-167 (2005).
J. Laskin. “Energetics and Dynamics of Peptide Fragmentation from Multiple-Collision Activation and Surface-Induced Dissociation Studies.” Eur. J. Mass Spectrom., Jean Futrell and Burnaby Munson Sp. Issue, 10, 259-267 (2004).
J. Laskin. “Energy and Entropy Effects in Gas-Phase Dissociation of Peptides and Proteins,” in Principles of Mass Spectrometry Applied to Biomolecules, J. Laskin and C. Lifshitz (Eds.), Wiley, Hoboken, NJ, 2006, pp. 619-665.
J. Laskin- “Fragmentation Energetics of Protonated Leucine Enkephalin from Time-and Energy-Resolved Surface-Induced Dissociation Studies”, J. Phys. Chem. A, 110, 8554-8562 (2006)
J. Laskin, K.M. Beck, J.J. Hache, J.H. Futrell. “Surface-Induced Dissociation of Ions Produced by Matrix-Assisted Laser Desorption Ionization in a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer.” Anal. Chem., 76, 351-356 (2004).
J. Laskin, T.H. Bailey and J.H. Futrell. “Fragmentation Energetics for Angiotensin II and its Analogs from Time-and Energy-Resolved Surface-Induced Dissociation Studies.” Int. J. Mass Spectrom., Alan Marshall Sp. Issue, 234, 89–99 (2004).
J. Laskin, T.H. Bailey and J.H. Futrell “Mechanisms of Peptide Fragmentation from Time-and Energy-Resolved Surface-Induced Dissociation Studies: Dissociation of Angiotensin Analogs,” Int. J. Mass Spectrom., Chava Lifshitz Memorial Issue, 249–250, 462–472 (2006)
Lloyd JA, JM Spraggins, MV Johnston, and J Laskin. 2006. "Peptide Ozonolysis: Product Structures and Relative Reactivities for Oxidation of Tyrosine and Histidine Residues ." Journal of the American Society for Mass Spectrometry 17(9):1289-1298.
Z. Yang, O. Hadjar and J. Laskin. “Effect of the Surface Morphology on the Energy Transfer in Ion-Surface Collisions,” Int. J. Mass Spectrom., Jean Futrell Honor Issue, 265, 124–129 (2007).