Activity-based probes (ABPs) are substrate-based, small organic compounds that enable the capture of the functional state of proteins in vivo and in vitro, isolation of cells with targeted functions, elucidation of biochemical pathways, and annotation of proteins of unknown function. Using advanced analytical tools, researchers gain spatio-temporal understanding of functional proteins in biochemical processes.

The Environmental Molecular Sciences Laboratory (EMSL) offers access to a non-canonical amide hydrolase probe library for novel functional discovery and protein annotation. EMSL users can profile cystine and serine proteases using broad spectrum serine and cystine protease probes. Novel probes can be custom developed to meet the specific needs of individual users.

Research Application

Supporting the Biomolecular Pathways Integrated Research Platform, ABPs allow probe-targeted proteomics analysis for differentiating functional states of enzymes in various samples. APBs are also used to annotate proteins of unknown function and phenotype specific cell isolation for further omics analyses. This includes cell-specific proteomics or single-cell RNA sequencing for transcriptional analysis. 

Supporting the Cell Signaling and Communication Integrated Research Platform, ABPs enable spatio-temporal understanding of enzymes within cell or in microbial communities at the cellular and molecular levels, tracking signaling and exchanging of metabolites within microbial communities.

Supporting the Rhizosphere Function Integrated Research Platform, ABPs provide new insights into host-microbe interactions and metabolite exchanges and their role in plant growth.

Tips for Success

Work with EMSL staff scientists to preserve the functional and biochemical activity of the proteins in the sample when shipping.