Confocal Raman Spectrometry
Confocal Raman microscopy (CRM) is an improved system within traditional Raman spectroscopy in that it provides chemical maps with down to < 1 mm spatial resolution and allows for the acquisition of depth profiles in three-dimensional thin structures. CRM couples a Raman spectrometer with a confocal Raman microscope, making it possible to analyze individual particles or layers with dimensions down to 1 µm and below. The main advantage of CRM over conventional microscopy is the enhanced depth resolution and the much higher image contrast due to stray light suppression. CRM’s enhanced spatial resolution combined with clear image quality and the lack of a sample preparation requirement enable EMSL researchers to easily conduct chemical and molecular analysis of multilayer samples.
Research application
- By analyzing a range of spatial scales in the identification and mapping of multilayer samples, the CRM supports the Terrestrial-Atmosphere Processes Integrated Research Platform by helping EMSL researchers uncover the mechanistic drivers and controls of pertinent environmental fluxes.
- CRM supports the Biogeochemical Transformations Integrated Research Platform by assisting EMSL researchers in answering fundamental questions about chemical, physical, and microbial interactions that affect the transformation and mobility of critical nutrients, particles, and compounds within the environment.
Tips for success
- CRM has minimal sample constraints and can be used on many different systems including plants, microbes, liquids, minerals, polymer/plastics, and many others.