Fluorescence-Activated Cell Sorting

The Fluorescence-Activated Cell Sorter (FACS) allows collection and enrichment of unique populations of cells, or single cells, from a heterogenous culture. Cells can be collected based on size, complexity, or fluorescent signals, such as those emitted by fluorescent proteins, organic dyes, or internal pigments. The detected information can then be used for the enrichment of specific populations or isolation of individual cells for propagation or omics analysis.

Research application

• Supporting the Biomolecular Pathways Integrated Research Platform, this resource enables the isolation of specific cell-types or single cells for further omics analyses, such as cell-specific proteomics or single-cell RNA-Seq for transcriptional analysis.  
• Supporting the Cell Signaling and Communication Integrated Research Platform, this resource supports studies aimed at better understanding the role of specific cells within tissues or microbial communities at the cellular and molecular levels.
• Supporting the Rhizosphere Function Integrated Research Platform, this resource provides new insights to the function of soil microbial communities and their role in plant growth.

Available instruments

• BD Influx Fluorescence-Activated Cell Sorter

Tips for success

• Samples must be in a single-cell suspension.
• Make sure that the fluorophores of interest are paired with one of the 5 excitation lasers and 13 emissions detection filters.