Raman atomic force microscopy (AFM) combines AFM and tip-enhanced Raman spectroscopy (TERS). Both are used for chemical imaging. AFM-Raman uses AFM to map the surface morphology with few-nanometer spatial resolution, while simultaneously recording co-located Raman spectra using confocal microscopy. This allows chemical imaging with few-hundred-nanometer spatial resolution via Raman spectroscopy. TERS is used for chemical imaging with a few-nanometer spatial resolution. This relies on signal enhancement from the interaction between a metallic surface and the metallic AFM tip. TERS can be performed in liquid environments and is used to track chemical reactions in situ.
- Supporting the Structural Biology Integrated Research Platform, these resources can image and identify bio-structures with nanoscale spatial resolution.
- Supporting the Biogeochemical Transformations Integrated Research Platform, these resources can track chemical changes in biogeochemical or hydrological systems.
Tips for success
- Samples of almost any form are compatible with AFM-Raman. However, samples provided in solution or suspension allow for chemical identification with the highest sensitivity.