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TerraForms: SubTap Microbial and RhizoTap Rhizosphere Platforms

SubTap Microbial and RhizoTap Rhizosphere Platforms
SubTap Microbial and RhizoTap Rhizosphere Platforms
SubTap Microbial and RhizoTap Rhizosphere Platforms
SubTap Microbial and RhizoTap Rhizosphere Platforms
SubTap Microbial and RhizoTap Rhizosphere Platforms
SubTap Microbial and RhizoTap Rhizosphere Platforms
SubTap Microbial and RhizoTap Rhizosphere Platforms

The SubTap and RhizoTap platforms are for rapid analysis of metabolites resulting from plant–microbe and microbe–microbe interactions. These platforms are in the format of 96 well plates and is for large-scale and high-throughput (HTP) interrogation of microbial and plant-exuded metabolites to enable screening of a range of interactions.

Through multiculture in spatially mixed or unmixed configurations, the SubTap platforms allow for rapid chemical phenotyping of several sets of plant–microbe interactions at a time. Both the SubTap microbial and SubTapRhizo (plant) platforms are 3D printed and can be customized for your research needs.

Research application

Supporting the Biogeochemical Transformations Integrated Research Platform, these resources help unlock how contaminants move and change in the environment. This essential knowledge can help us improve predictive models to anticipate and manage effects on both human and natural systems. EMSL’s microfluidic and 3D printed technologies allow scientists to create TerraForms to understand and to study microbe–mineral and microbe–plant root interactions.

Supporting the Rhizosphere Function Integrated Research Platform, TerraForms enables researchers to study plant-root–microbe interactions.

Supporting the Terrestrial-Atmospheric Processes Integrated Research Platform, TerraForms enables identifying belowground and aboveground volatile organic compounds (VOCs) for your science.

Available instruments

Tips for success

Request these platforms for rapid phenotyping, metabolomics, and genotyping of microbes and the plant rhizosphere. These are also compatible with investigation of VOCs. Reach out to Arunima Bhattacharjee for accessing the SubTap platforms. Design changes can be made per user research requirements.

Contributing teams and resources 

EMSL develops and deploys capabilities for the user program by conducting original research independently or in partnership with others and by adapting/advancing science and technologies developed outside of EMSL. In some instances, EMSL directly deploys mature capabilities developed by others where there is value for the EMSL user community. The following grants/activities, PI’s and teams contributed to the development of this capability: 

  • Eric Wright, NIAID grant 1DP2AI145058-01, National Institute of Health
  • Arunima Bhattacharjee, DE-AC05-76RL01830, Environmental Molecular Sciences Laboratory

Related Publication

Birer-Williams, C, et al. "SubTap, a Versatile 3D Printed Platform for Eavesdropping on Extracellular Interactions." mSystems. Vol. 6, No. 4. (2021) [DOI: 10.1128/msystems.00902-21]

 

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