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Rosalie Chu

Rosalie Chu is a chemist with the Biomolecular Pathways team. She is an expert in high-resolution mass spectrometry (MS), including Fourier transform ion cyclotron resonance MS and linear ion trapping instrumentation relevant to complex mixtures. Chu is proficient in sample preparation, instrument maintenance, instrumentation/data acquisition, method development, data analysis, and visualization.  

Chu has experience in reverse-phase and HILIC LC-MS operation and separation optimization for the identification of peptides, lipids, and small molecules. She also has experience in MS imaging (MSI) techniques that include matrix assisted laser desorption/ionization and liquid extracted surface analysis for lipids and metabolites. She is also familiar with ion chromatography and inductively coupled plasma-MS. 

Chu helps principal investigators from various institutions to design experiments through data interpretation. In addition to PNNL and EMSL, she has worked at Oregon Health Sciences University, Intel Corporation, Coffey Laboratories, and Kanto Corp. 

Research Interests

  • Mass Spectrometry 

  • Analytical Chemistry 


  • BS in Biology with a minor in Chemistry, University of Oregon, 1995 



Chaput G., A. Billings, L. DeDiego, R. Orellana, J.N. Adkins, C.D. Nicora, and Y. Kim, et al. 2020. "Lignin Induced Iron Reduction by Novel sp., Tolumonas lignolytic BRL6-1." PLOS ONE 15, no. 9:e0233823. PNNL-SA-156112. doi:10.1371/journal.pone.0233823 

Dou Y., E. Kawaler, D.C. Zhou, M.A. Gritsenko, C. Huang, L. Blumenberg, and A. Karpova, et al. 2020. "Proteogenomic Characterization of Endometrial Carcinoma." Cell 180, no. 4:729-748.e26. PNNL-SA-147326. doi:10.1016/j.cell.2020.01.026 

Garayburu-Caruso V.A., J.C. Stegen, H. Song, L. Renteria, J.R. Wells, W.L. Garcia, and C.T. Resch, et al. 2020. "Carbon limitation leads to thermodynamic regulation of aerobic metabolism." Environmental Science & Technology Letters 7, no. 7:517-524. PNNL-SA-150146. doi:10.1021/acs.estlett.0c00258 

Hawkes J., J. D'Andrilli, J.N. Agar, M. Barrow, S. Berg, N. Catalan, and H. Chen, et al. 2020. "An international laboratory comparison of dissolved organic matter composition by high resolution mass spectrometry: Are we getting the same answer?" Limnology and Oceanography Methods 18, no. 6:235-258. PNNL-SA-147033. doi:10.1002/lom3.10364 

Morrison, E. S., M. R. Shields, T. S. Bianchi, Y. Liu, S. Newman, N. Tolic, and R. K. Chu. 2020. “Multiple Biomarkers Highlight the Importance of Water Column Processes in Treatment Wetland Organic Matter Cycling.” Water Research 168.  

Veličković, D., R. K. Chu, G. L. Myers, A. H. Ahkami, and C. R. Anderton. 2020. “An Approach for Visualizing the Spatial Metabolome of an Entire Plant Root System Inspired by the Swiss-Rolling Technique.” Journal of Mass Spectrometry 55 (4).  

Xu J., S. Roley, M. Tfaily, R.K. Chu, and J.M. Tiedje. 2020. "Organic amendments change soil organic C structure and microbial community but not total organic matter on sub-decadal scales." Soil Biology and Biochemistry 150. PNNL-SA-155812. doi:10.1016/j.soilbio.2020.107986 

Yang, J., H. Jiang, W. Liu, L. Huang, J. Huang, B. Wang, H. Dong, R. K. Chu, and N. Tolic. 2020. “Potential Utilization of Terrestrially Derived Dissolved Organic Matter by Aquatic Microbial Communities in Saline Lakes.” ISME Journal 14 (9): 2313-2324.  


Chacon S.S., P.N. Reardon, C.J. Burgess, S.O. Purvine, R.K. Chu, T.R. Clauss, and E.D. Walter, et al. 2019. "Mineral Surfaces as Agents of Environmental Proteolysis: Mechanisms and Controls." Environmental Science & Technology 53, no. 6:3018-3026. PNNL-SA-141783. doi:10.1021/acs.est.8b05583 

Fudyma J.D., J.E. Lyon, R. Aminitabrizi, H. Gieschen, R.K. Chu, D.W. Hoyt, and J.E. Kyle, et al. 2019. "Untargeted metabolomic profiling of Sphagnum fallax reveals novel antimicrobial metabolites." Plant Direct 3, no. 11:Article No. e00179. PNNL-SA-146621. doi:10.1002/pld3.179 

Nagy G., D. Velickovic, R.K. Chu, A.A. Carrell, D.J. Weston, Y.M. Ibrahim, and C.R. Anderton, et al. 2019. "Towards Resolving the Spatial Metabolome with Unambiguous Molecular Annotations in Complex Biological Systems by Coupling Mass Spectrometry Imaging with Structures for Lossless Ion Manipulations." Chemical Communications 55, no. 3:306-309. PNNL-SA-138045. doi:10.1039/C8CC07482H 

Sengupta, A., J. Indivero, C. Gunn, M. M. Tfaily, R. K. Chu, J. Toyoda, V. L. Bailey, N. D. Ward, and J. C. Stegen. 2019. “Spatial Gradients in the Characteristics of Soil-Carbon Fractions Are Associated with Abiotic Features but Not Microbial Communities.” Biogeosciences 16 (19): 3911-3928.  

Tfaily M., R.M. Wilson, H.M. Brewer, R.K. Chu, H.M. Heyman, D.W. Hoyt, and J.E. Kyle, et al. 2019. "Single-throughput complementary high-resolution analytical techniques for characterizing complex natural organic matter mixtures." Journal of Visualized Experiments. PNNL-SA-138197. doi:10.3791/59035 

Velickovic D., H. Liao, R. Vilgalys, R.K. Chu, and C.R. Anderton. 2019. "Spatiotemporal Transformation in the Alkaloid Profile of Pinus Roots in Response to Mycorrhization." Journal of Natural Products 82, no. 5:1382-1386. PNNL-SA-140336. doi:10.1021/acs.jnatprod.8b01050 

Ward A.S., S.M. Wondzell, N.M. Schmadel, S. Herzog, J.P. Zarnetske, V. Baranov, and P. Blaen, et al. 2019. "Spatial and temporal variation in river corridor exchange across a 5th order mountain stream network." Hydrology and Earth System Sciences 23, no. 12:5199-5225. PNNL-SA-140824. doi:10.5194/hess-23-5199-2019 

Ward, A. S., J. P. Zarnetske, V. Baranov, P. J. Blaen, N. Brekenfeld, R. Chu, R. Derelle, J. Drummond, J. H. Fleckenstein, V. Garayburu-Caruso, E. Graham, D. Hannah, C. J. Harman, S. Herzog, J. Hixson, J. L. A. Knapp, S. Krause, M. J. Kurz, J. Lewandowski, A. Li, E. Marti, M. Miller, A. M. Milner, K. Neil, L. Orsini, A. I. Packman, S. Plont, L. Renteria, K. Roche, T. Royer, N. M. Schmadel, C. Segura, J. Stegen, J. Toyoda, J. Wells, N. I. Wisnoski, and S. M. Wondzell. 2019. “Co-Located Contemporaneous Mapping of Morphological, Hydrological, Chemical, and Biological Conditions in a 5th-Order Mountain Stream Network, Oregon, USA.” Earth System Science Data 11 (4): 1567-1581.  

Yi L., C. Tsai, E. Dirice, A.C. Swensen, J. Chen, T. Shi, and M.A. Gritsenko, et al. 2019. "Boosting to Amplify Signal with Isobaric Labeling (BASIL) Strategy for Comprehensive Quantitative Phosphoproteomic Characterization of Small Populations of Cells." Analytical Chemistry 91, no. 9:5794-5801. PNNL-SA-140381. doi:10.1021/acs.analchem.9b00024 

Zhou, Z., T. Yu, H. Dong, L. Huang, R. K. Chu, N. Tolic, X. Wang, and Q. Zeng. 2019. “Chemical Oxygen Demand (COD) Removal from Bio-Treated Coking Wastewater by Hydroxyl Radicals Produced from a Reduced Clay Mineral.” Applied Clay Science 180.  


Bottos E.M., D.W. Kennedy, E.B. Romero, S.J. Fansler, J.M. Brown, L.M. Bramer, and R.K. Chu, et al. 2018. "Dispersal Limitation and Thermodynamic Constraints Govern Spatial Structure of Permafrost Microbial Communities." FEMS Microbiology Ecology 94, no. 8:fiy110. PNNL-SA-130968. doi:10.1093/femsec/fiy110 

Chen H., Z. Yang, R.K. Chu, N. Tolic, L. Liang, D.E. Graham, and S.D. Wullschleger, et al. 2018. "Molecular Insights into Arctic Soil Organic Matter Degradation under Warming." Environmental Science & Technology 52, no. 8:4555-4564. PNNL-SA-136151. doi:10.1021/acs.est.7b05469 

Duncan K.D., R. Fang, J. Yuan, R.K. Chu, S.K. Dey, K.E. Burnum-Johnson, and I. Lanekoff. 2018. "Quantitative mass spectrometry imaging of prostaglandins as silver ion adducts with nanospray desorption electrospray ionization." Analytical Chemistry 90, no. 12:7246-7252. PNNL-SA-131518. doi:10.1021/acs.analchem.8b00350 

Liang Y., Y. Zhu, M. Dou, K. Xu, R.K. Chu, W.B. Chrisler, and R. Zhao, et al. 2018. "Spatially resolved proteomic profiling of <200 cells from tomato fruit pericarp by integrating laser-capture microdissection with nanodroplet sample preparation." Analytical Chemistry 90, no. 18:11106-11114. PNNL-SA-134717. doi:10.1021/acs.analchem.8b03005 

Orton D.J., M.M. Tfaily, R.J. Moore, B.L. Lamarche, X. Zheng, T.L. Fillmore, and R.K. Chu, et al. 2018. "A Customizable Flow Injection System for Automated, High Throughput and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements." Analytical Chemistry 90, no. 1:737-744. PNNL-SA-128111. doi:10.1021/acs.analchem.7b02986 

Stegen, J. C., T. Johnson, J. K. Fredrickson, M. J. Wilkins, A. E. Konopka, W. C. Nelson, E. V. Arntzen, W. B. Chrisler, R. K. Chu, S. J. Fansler, E. B. Graham, D. W. Kennedy, C. T. Resch, M. Tfaily, and J. Zachara. 2018. “Influences of Organic Carbon Speciation on Hyporheic Corridor Biogeochemistry and Microbial Ecology.” Nature Communications 9 (1).  

Stegen, J. C., T. Johnson, J. K. Fredrickson, M. J. Wilkins, A. E. Konopka, W. C. Nelson, E. V. Arntzen, W. B. Chrisler, R. K. Chu, S. J. Fansler, E. B. Graham, D. W. Kennedy, C. T. Resch, M. Tfaily, and J. Zachara. 2018. “Publisher Correction: Influences of Organic Carbon Speciation on Hyporheic Corridor Biogeochemistry and Microbial Ecology (Nature Communications (2018) doi: 10.1038/S41467-018-02922-9).” Nature Communications 9 (1).  

Velickovic D., R.K. Chu, A.A. Carrell, M. Thomas, L. Pasa Tolic, D.J. Weston, and C.R. Anderton. 2018. "Multimodal MSI in Conjunction with Broad Coverage Spatially Resolved MS2 Increases Confidence in Both Molecular Identification and Localization." Analytical Chemistry 90, no. 1:702-707. PNNL-SA-132227. doi:10.1021/acs.analchem.7b04319 

Zhu, Y., M. Dou, P. D. Piehowski, Y. Liang, F. Wang, R. K. Chu, W. B. Chrisler, J. N. Smith, K. C. Schwarz, Y. Shen, A. K. Shukla, R. J. Moore, R. D. Smith, W. J. Qian, and R. T. Kelly. 2018. “Spatially Resolved Proteome Mapping of Laser Capture Microdissected Tissue with Automated Sample Transfer to Nanodroplets.” Molecular and Cellular Proteomics 17 (9): 1864-1874.  


Blair S.L., A.C. Macmillan, G.T. Drozd, A.H. Goldstein, R.K. Chu, L. Pasa Tolic, and J.B. Shaw, et al. 2017. "Molecular Characterization of Organosulfur Compounds in Biodiesel and Diesel Fuel Secondary Organic Aerosol." Environmental Science & Technology 51, no. 1:119-127. PNNL-SA-119437. doi:10.1021/acs.est.6b03304 

Chen, H., R. C. Johnston, B. F. Mann, R. K. Chu, N. Tolic, J. M. Parks, and B. Gu. 2017. “Identification of Mercury and Dissolved Organic Matter Complexes Using Ultrahigh Resolution Mass Spectrometry.” Environmental Science and Technology Letters 4 (2): 59-65.  

Cook R.D., Y. Lin, Z. Peng, E.J. Boone, R.K. Chu, J.E. Dukett, and M.J. Gunsch, et al. 2017. "Biogenic, urban, and wildfire influences on the molecular composition of dissolved organic compounds in cloud water." Atmospheric Chemistry and Physics 17, no. 24:15167-15180. PNNL-SA-130575. doi:10.5194/acp-17-15167-2017 

Dohnalkova A., M.M. Tfaily, A.P. Smith, R.K. Chu, A.R. Crump, C.J. Brislawn, and T. Varga, et al. 2017. "Molecular and Microscopic Insights into the Formation of Soil Organic Matter in a Red Pine Rhizosphere." Soils 1, no. 1:Article No. 4. PNNL-SA-128886. doi:10.3390/soils1010004 

Nguyen S.N., A.V. Liyu, R.K. Chu, C.R. Anderton, and J. Laskin. 2017. "Constant-Distance Mode Nanospray Desorption Electrospray Ionization Mass Spectrometry Imaging of Biological Samples of Complex Topography." Analytical Chemistry 89, no. 2:1131-1137. PNNL-SA-119825. doi:10.1021/acs.analchem.6b03293 

Staley C., A.P. Ferrieri, M.M. Tfaily, Y. Cui, R.K. Chu, P. Wang, and J.B. Shaw, et al. 2017. "Diurnal Cycling of Rhizosphere Bacterial Communities is Associated with Shifts in Carbon Metabolism." Microbiome 5. PNNL-SA-121718. doi:10.1186/s40168-017-0287-1 

Tfaily M.M., R.K. Chu, J.G. Toyoda, N. Tolic, E.W. Robinson, L. Pasa Tolic, and N.J. Hess. 2017. "Sequential extraction protocol for organic matter from soils and sediments using high resolution mass spectrometry." Analytica Chimica Acta 972. PNNL-SA-125053. doi:10.1016/j.aca.2017.03.031 

Truex M.J., B.D. Lee, C.D. Johnson, N. Qafoku, J.E. Szecsody, J.E. Kyle, and M.M. Tfaily, et al. 2017. Conceptual Model of Iodine Behavior in the Subsurface at the Hanford Site. PNNL-24709 Rev. 2. Richland, WA: Pacific Northwest National Laboratory. Conceptual Model of Iodine Behavior in the Subsurface at the Hanford Site 


Anderton C.R., R.K. Chu, N. Tolic, A.V. Creissen, and L. Pasa-Tolic. 2016. "Utilizing a Robotic Sprayer for High Lateral and Mass Resolution MALDI FT-ICR MSI of Microbial Cultures." Journal of the American Society for Mass Spectrometry 27, no. 3:556-559. PNNL-SA-115142. doi:10.1007/s13361-015-1324-6 

Duan, J., V. K. Kodali, M. J. Gaffrey, J. Guo, R. K. Chu, D. G. Camp, R. D. Smith, B. D. Thrall, and W. J. Qian. 2016. “Quantitative Profiling of Protein S-Glutathionylation Reveals Redox-Dependent Regulation of Macrophage Function During Nanoparticle-Induced Oxidative Stress.” ACS Nano 10 (1): 524-538.  

Nakayasu E.S., C.D. Nicora, A.C. Sims, K.E. Burnum-Johnson, Y. Kim, J.E. Kyle, and M.M. Matzke, et al. 2016. "MPLEx: a Robust and Universal Protocol for Single-Sample Integrative Proteomic, Metabolomic, and Lipidomic Analyses." mSystems 1, no. 3:e00043-16. PNNL-SA-114737. doi:10.1128/mSystems.00043-16 

Pomraning K.R., Y. Kim, C.D. Nicora, R.K. Chu, E.L. Bredeweg, S.O. Purvine, and D. Hu, et al. 2016. "Multi-omics analysis reveals regulators of the response to nitrogen limitation in Yarrowia lipolytica." BMC Genomics 17, no. 138:1-18. PNNL-SA-112784. doi:10.1186/s12864-016-2471-2 

Stegen J.C., A.H. Hurlbert, B. Bond-Lamberty, X. Chen, C.G. Anderson, R.K. Chu, and F. Dini-Andreote, et al. 2016. "Aligning the Measurement of Microbial Diversity with Macroecological Theory." Frontiers in Microbiology 7. PNNL-SA-122085. doi:10.3389/fmicb.2016.01487 

Stegen J.C., J.K. Fredrickson, M.J. Wilkins, A. Konopka, W.C. Nelson, E.V. Arntzen, and W.B. Chrisler, et al. 2016. "Groundwater-Surface Water Mixing Shifts Ecological Assembly Processes and Stimulates Organic Carbon Turnover." Nature Communications 7. PNNL-SA-111962. doi:10.1038/ncomms11237 

Sun J., T. Dutta, R. Parthasarathi, K. Kim, N. Tolic, R.K. Chu, and N.G. Isern, et al. 2016. "Rapid room temperature solubilization and depolymerization of polymeric lignin at high loadings." Green Chemistry 18, no. 22:6012-6020. PNNL-SA-120307. doi:10.1039/C6GC02258H 

Tolic N., M.M. Tfaily, Y. Shen, R.K. Chu, T.L. Fillmore, R. Zhao, and K.J. Bloodsworth, et al. 2016. "Soil Organic Matter (SOM): Mass Spectrometry Data Analysis." In Encyclopedia of Soil Science, Third Edition, edited by R Lal. Boca Raton, Florida:CRC Press. PNNL-SA-117244. doi:10.1081/E-ESS3-120053757 


Duan J., V.K. Kodali, M.J. Gaffrey, J. Guo, R.K. Chu, D.G. Camp, and R.D. Smith, et al. 2015. "Quantitative Profiling of Protein S-Glutathionylation Reveals Redox-Dependent Regulation of Macrophage Function During Nanoparticle-Induced Oxidative Stress." ACS Nano 10, no. 1:524-538. PNNL-SA-112721. doi:10.1021/acsnano.5b05524 

Kebaabetswe L.P., A.K. Haick, M.A. Gritsenko, T.L. Fillmore, R.K. Chu, S.O. Purvine, and B.M. Webb-Robertson, et al. 2015. "Proteomic Analysis Reveals Down-Regulation of Surfactant Protein B in Murine Type II Pneumocytes Infected with Influenza A Virus." Virology 438. PNWD-SA-10439. doi:10.1016/j.virol.2015.03.045 

Mann B.F., H. Chen, E.M. Herndon, R.K. Chu, N. Tolic, E. Portier, and T.R. Chowdhury, et al. 2015. "Indexing Permafrost Soil Organic Matter Degradation Using High-Resolution Mass Spectrometry." PLoS One 10, no. 6:Article No. e0130557. PNNL-SA-111173. doi:10.1371/journal.pone.0130557 

Tfaily M.M., R.K. Chu, N. Tolic, K.M. Roscioli, C.R. Anderton, L. Pasa-Tolic, and E.W. Robinson, et al. 2015. "Advanced solvent based methods for molecular characterization of soil organic matter by high-resolution mass spectrometry." Analytical Chemistry 87, no. 10:5206-5215. PNNL-SA-107718. doi:10.1021/acs.analchem.5b00116 


Dang X., J. Scotcher, S. Wu, R.K. Chu, N. Tolic, I. Ntai, and P. Thomas, et al. 2014. "The first pilot project of the consortium for top-down proteomics: A status report." Proteomics 14, no. 10:1130-1140. PNNL-SA-102713. doi:10.1002/pmic.201300438 

Hyung S., P.D. Piehowski, R.J. Moore, D.J. Orton, A.A. Schepmoes, T.R. Clauss, and R.K. Chu, et al. 2014. "Microscale Depletion of High Abundance Proteins in Human Biofluids using IgY14 Immunoaffinity Resin: Analysis of Human Plasma and Cerebrospinal Fluid." Analytical and Bioanalytical Chemistry 406, no. 28:7117-7125. PNNL-SA-92758. doi:10.1007/s00216-014-8058-3 

Su D., M.J. Gaffrey, J. Guo, K.E. Hatchell, R.K. Chu, T.R. Clauss, and J.T. Aldrich, et al. 2014. "Proteomic Identification and Quantification of S-glutathionylation in Mouse Macrophages Using Resin-Assisted Enrichment and Isobaric Labeling." Free Radical Biology & Medicine 67. PNNL-SA-96702. doi:10.1016/j.freeradbiomed.2013.12.004 


Venceslau S.S., J.R. Cort, E.S. Baker, R.K. Chu, E.W. Robinson, C. Dahl, and L.M. Saraiva, et al. 2013. "Redox states of Desulfovibrio vulgaris DsrC, a key protein in dissimilatory sulfite reduction." Biochemical and Biophysical Research Communications 441, no. 4:731-736. PNNL-SA-98016. doi:10.1016/j.bbrc.2013.10.116 


Gao X., Q. Zhang, D. Meng, G. Isaac, R. Zhao, T.L. Fillmore, and R.K. Chu, et al. 2012. "A reversed-phase capillary ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) method for comprehensive top-down/bottom-up lipid profiling." Analytical and Bioanalytical Chemistry 402, no. 9:2923-2933. PNWD-SA-9553.