Steven Hallam

Mass-spectral 'fingerprinting' of technical and native lignin substrates for improved biological funneling and enzyme discovery

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Large-Scale EMSL Research

Lignin, composed of phenylpropanoid monomers connected by C-C and C-O-C (β-O-4 ether) bonds is the most energy dense polymer in lignocellulosic biomass and a potentially valuable source of aromatic…

Microbial controls on climate active trace gas cycling in a model oxygen minimum zone

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Large-Scale EMSL Research

Dissolved oxygen (O2) concentration is a critical organizing principle within marine ecosystems. As oxygen levels decline, energy is increasingly diverted away from higher trophic levels into…

Microbial controls on climate active trace gas cycling in forest soil ecosystems

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Large-Scale EMSL Research

Globally, soils store more than double the C in the planet’s atmosphere, and three times the C in aboveground biomass. This large carbon reservoir, stored as soil organic matter (SOM), can be…

Engineering enzyme networks for biomass deconstruction in synthetic microbial communities

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

FICUS Research

Lignocellulose, the main component of plant cell walls, is arguably the most abundant biopolymer on the planet1,2. Through biorefining, it is also a renewable input for energy and materials…

Expanding the functional metagenomics toolkit with CRAGE and CRISPR-Cas9 in taxonomically diverse microbial host chassis

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

FICUS Research

Plant and microbial biomass offers a renewable alternative to energy and materials produced from oil. The industrial scale production or biorefining of sugars and aromatic compounds from biomass…

Protemic Analysis of Naturally Ocurring Methane Oxidizing Archaeal Communities

Lead Institution

Massachusetts Institute of Technology

Principal Investigator

Edward DeLong

Project type

Exploratory Research

Microbial mediated anaerobic methane oxidation (AOM) in marine sediments represents a significant but poorly understood carbon sink throughout the world's oceans. Mounting geochemical and…

Tracking carbon flow through syntrophic interspecies interactions in methanogenic microbiomes

Lead Institution

University of British Columbia

Principal Investigator

Ryan Ziels

Project type

Large-Scale EMSL Research

Determining in situ metabolic processes and interactions among diverse assemblages of microorganisms remains a critical challenge in microbial ecology. Consequently, identifying novel genes and…

Resolving carbon fluxes within microbial communities inhabiting full-scale bioenergy facilities

Lead Institution

University of British Columbia

Principal Investigator

Ryan Ziels

Project type

FICUS Research

Elucidating the physiology and metabolic function of uncultivated microorganisms in their in situ environment remains a critical challenge in microbial ecology. This knowledge gap obfuscates attempts…

Environmental proteomic analysis of anaerobic methane oxidizing systems

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Large-Scale EMSL Research

Prokaryotic microorganisms represent the majority of living things on Earth. In many cases, interdependent constituents of this vast microbial biota play decisive roles in defining biogeochemical…

Comparative Proteomic Analysis of Naturally Occurring Anaerobic Methane Oxidizing Communities

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Large-Scale EMSL Research

Methane is a potent greenhouse gas whose atmospheric concentration has increased significantly due to anthropogenic activities and fluctuated naturally over glacial and interglacial cycles. While…

Community Proteomic Analysis of a Near-Shore Anoxic Basin: Saanich Inlet

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Large-Scale EMSL Research

Oxygen minimum zones (OMZs; areas of low dissolved oxygen concentrations) play a major role in biogeochemical cycling within the world's oceans. They are major sinks for nitrogen and sources for…

Microbial Systems Ecology of a Seasonally Anoxic Fjord: Saanich Inlet

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Large-Scale EMSL Research

Marine oxygen minimum zones (OMZs) are widespread oceanographic features currently expanding due to global warming. Although inhospitable to aerobic respiring organisms, OMZs support a thriving…

Reverse Methanogenesis: Validating the Hypothesis with Lineage-Resolved Environmental Proteomics

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Large-Scale EMSL Research

In marine sediments three known lineages of anaerobic methane oxidizing archaea (ANME-1, 2 and 3) filter up to ~384 Tg of the greenhouse gas methane annually, greatly reducing methane flux from ocean…

Development of the Integrated MetaPROteomics Viewer (IMPROV) Software Toolkit

Lead Institution

University of British Columbia

Principal Investigator

Steven Hallam

Project type

Scientific Partner

Beyond the periphery of our senses exists an infinitely diverse microbial world. This microcosm extends into every imaginable habitat, thrives under powerful extremes, and helps create and sustain…

EMSL Science Overview

Steven Hallam

Projects

Mass-spectral 'fingerprinting' of technical and native lignin substrates for improved biological funneling and enzyme discovery

Microbial controls on climate active trace gas cycling in a model oxygen minimum zone

Microbial controls on climate active trace gas cycling in forest soil ecosystems

Engineering enzyme networks for biomass deconstruction in synthetic microbial communities

Expanding the functional metagenomics toolkit with CRAGE and CRISPR-Cas9 in taxonomically diverse microbial host chassis

Protemic Analysis of Naturally Ocurring Methane Oxidizing Archaeal Communities

Tracking carbon flow through syntrophic interspecies interactions in methanogenic microbiomes

Resolving carbon fluxes within microbial communities inhabiting full-scale bioenergy facilities

Environmental proteomic analysis of anaerobic methane oxidizing systems

Comparative Proteomic Analysis of Naturally Occurring Anaerobic Methane Oxidizing Communities

Community Proteomic Analysis of a Near-Shore Anoxic Basin: Saanich Inlet

Microbial Systems Ecology of a Seasonally Anoxic Fjord: Saanich Inlet

Reverse Methanogenesis: Validating the Hypothesis with Lineage-Resolved Environmental Proteomics

Development of the Integrated MetaPROteomics Viewer (IMPROV) Software Toolkit