Victoria Orphan

Novel Multimodal Chemical Nano-Imaging Approach to Visualize and Identify Small Biomolecules Exchanged in Microbial Communities

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Lea

The ability to chemically visualize biological systems and processes on the nanometer length scale under relevant physiological conditions is an unmet need that if realized would revolutionize our…

Fluorescence-based cell sorting and targeted proteomic analysis of active methane-oxidizing syntrophic consortia from environmental samples

Lead Institution

California Institute of Technology

Principal Investigator

Victoria Orphan

Project type

FICUS Research

Determining the links between phylogenetically identified microorganisms with their metabolic activity and function in complex natural ecosystems has been a long-standing challenge in microbial…

Characterizing the ecophysiologies of uncultured syntrophic associations driving anaerobic methane oxidation: applications of targeted meta'omics and multi-modal analytical imaging of diverse archaeal-bacterial consortia

Lead Institution

California Institute of Technology

Principal Investigator

Victoria Orphan

Project type

FICUS Research

The main objective of this work is to combine the molecular, elemental and cryo-electron imaging and metaproteomics available at EMSL with the single cell genomics pipeline at the JGI in a highly…

Multimodal Chemical Imaging Across Scales to Visualize Metabolic Pathways in Live Plants and Microbial Systems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Lea

We will develop a novel spectral imaging platform (“BioImager”) that may be used to image and identify biomolecules involved in both microbial and plant metabolic processes through different chemical…

Multimodal Chemical Imaging Across Scales to Visualize Metabolic Pathways in Live Plants and Microbial Systems

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Lea

We will develop a novel spectral imaging platform (“BioImager”) that may be used to image and identify biomolecules involved in both microbial and plant metabolic processes through different chemical…

EMSL Science Overview

Victoria Orphan

Projects

Novel Multimodal Chemical Nano-Imaging Approach to Visualize and Identify Small Biomolecules Exchanged in Microbial Communities

Fluorescence-based cell sorting and targeted proteomic analysis of active methane-oxidizing syntrophic consortia from environmental samples

Characterizing the ecophysiologies of uncultured syntrophic associations driving anaerobic methane oxidation: applications of targeted meta'omics and multi-modal analytical imaging of diverse archaeal-bacterial consortia

Multimodal Chemical Imaging Across Scales to Visualize Metabolic Pathways in Live Plants and Microbial Systems

Multimodal Chemical Imaging Across Scales to Visualize Metabolic Pathways in Live Plants and Microbial Systems