Grant Jensen

Collaborative Access Team: Pacific Northwest Cryo-EM Center (PNCC)

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Evans

PNNL will create and operate the Pacific Northwest Center for Cryo-EM (PNCC), a state-of-the-art national center for cryo-electron microscopy (Cryo-EM) that will rapidly and efficiently collect and…

Looking at the high-resolution structure of IRE1 inside cells using sub-tomogram averaging

Lead Institution

California Institute of Technology

Principal Investigator

Grant Jensen

The primary objective of our proposal is to image IRE1 foci using high-resolution cellular electron cryo-tomography (ECT). IRE1 is a member of the unfolded protein response (UPR). The UPR is an…

Investigating the cytoplasmic domain architecture of the Legionella pneumophila type IV secretion system using sub-tomogram averaging

Lead Institution

California Institute of Technology

Principal Investigator

Grant Jensen

In fresh water environment, Legionella pneumophila relies on its Dot/Icm type IV secretion system (T4SS) to translocate more than 300 effector proteins into its protozoan host. This process is…

Dissecting bacterial secretion systems in situ with electron cryo-tomography

Lead Institution

California Institute of Technology

Principal Investigator

Grant Jensen

Pathogenic bacteria use specialized “nanomachines” to identify and interact with host cells. These machines are attractive drug targets because they are surface-exposed, widespread, and vital for…

EMSL Science Overview

Grant Jensen

Projects

Collaborative Access Team: Pacific Northwest Cryo-EM Center (PNCC)

Looking at the high-resolution structure of IRE1 inside cells using sub-tomogram averaging

Investigating the cytoplasmic domain architecture of the Legionella pneumophila type IV secretion system using sub-tomogram averaging

Dissecting bacterial secretion systems in situ with electron cryo-tomography