James Fulcher

Unlocking the molecular basis of plant-pathogen interactions to create resilient bioenergy crops

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

In the winning 2023-DOE BRAVE (Biopreparedness Research Virtual Environment) Proposal (LAB-23-2955), awarded to co-PI Ljiljana Pasa-Tolic (EMSL/PNNL), PI Qun Liu (BNL), and other co-PI:, Clint Magill…

Spatially resolved characterization of proteoforms for functional proteomics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Differentiated cells have distinctive patterns of epigenetic marks including various post-translational modifications (PTMs) on histones that may work in concert to control transcriptional programs…

Proteome Changes Underlying Peripheral Sympathetic Dysfunction in Cardiac Disease

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Fulcher

Project Summary Myocardial infarction and heart failure increase risk for ventricular arrhythmias and sudden cardiac death. Autonomic dysregulation and sympathetic hyperactivity accompany these…

Hair Bundle Structure and Dynamics

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

James Fulcher

A principal long-term goal of our lab is to describe the molecular steps leading to hair-bundle assembly. As the mechanosensitive organelle of a hair cell, a bundle responds to mechanical forces by…

Nanodroplet sampling processing platform for amine containing metabolites in single Yarrowia lipolytical fungal protoplasts

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Sarah Williams

Single-cell metabolomics of bioproduction organisms such as Yarrowia lipolytica could significantly enhance our understanding of functional heterogeneity, yet remains a challenge due to limited input…

Massive single-cell proteomics for cancer biology

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

Single-cell technologies have become the cornerstone of biomedical and cell biology research. Next- generation sequencing-based technologies have enabled large-scale characterization of transcript…

High throughput single cell multiomics platform

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Ljiljana Pasa-Tolic

In this collaborative project, PNNL and Scienion will co-develop an integrated microfluidic technology to co-measure the transcriptome and proteome in single cells. The technology will enable us to…

Pathways and Critical Regulators of Early Beta-cell Dysfunction in Type 1 Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Type 1 diabetes (T1D) is a complex autoimmune disease. Recent clinical studies show that pancreatic beta-cell dysfunction (e.g., a decline of first phase insulin release) occurs several years before…

Pathway Discovery, Validation and Compound Identification for Alzheimer's Disease

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vladislav Petyuk

The overall goal of the proposed study is the discovery and preclinical validation of novel targets associated with molecular processes that lead to cognitive decline, the primary outcome of most AD…

Pathways and Critical Regulators of Early Beta-cell Dysfunction in Type 1 Diabetes

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Weijun Qian

Type 1 diabetes (T1D) is a complex autoimmune disease. Recent clinical studies show that pancreatic beta-cell dysfunction (e.g., a decline of first phase insulin release) occurs several years before…

Pathway Discovery, Validation and Compound Identification for Alzheimer's Disease

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Vladislav Petyuk

The overall goal of the proposed study is the discovery and preclinical validation of novel targets associated with molecular processes that lead to cognitive decline, the primary outcome of most AD…

Engineering Synthetic Anaerobic Consortia Inspired by the Rumen for Biomass Breakdown and Conversion

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Scott Baker

Lignocellulosic plant biomass is a widely-abundant renewable resource that can be harnessed for value-added production of fuels & chemicals. While microbes have been engineered to breakdown…

EMSL Science Overview

James Fulcher

Projects

Unlocking the molecular basis of plant-pathogen interactions to create resilient bioenergy crops

Spatially resolved characterization of proteoforms for functional proteomics

Proteome Changes Underlying Peripheral Sympathetic Dysfunction in Cardiac Disease

Hair Bundle Structure and Dynamics

Nanodroplet sampling processing platform for amine containing metabolites in single Yarrowia lipolytical fungal protoplasts

Massive single-cell proteomics for cancer biology

High throughput single cell multiomics platform

Pathways and Critical Regulators of Early Beta-cell Dysfunction in Type 1 Diabetes

Pathway Discovery, Validation and Compound Identification for Alzheimer's Disease

Pathways and Critical Regulators of Early Beta-cell Dysfunction in Type 1 Diabetes

Pathway Discovery, Validation and Compound Identification for Alzheimer's Disease

Engineering Synthetic Anaerobic Consortia Inspired by the Rumen for Biomass Breakdown and Conversion