Jesse Wilson

High-throughput native mass spectrometry as experimental validation for in silico drug design

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Enzyme functions can often be modulated via interaction with small molecules. For example, inhibitors are actively sought in the pharmaceutical industry to block protein targets and treat diseases…

Development of an ECD cell for biomolecular characterization

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Jared Shaw

The ultimate goal of this proposal is to integrate an electron capture dissociation (ECD) cell into ThermoFisher Scientific’s Exactive series mass spectrometers. The proposed work in collaboration…

Manganese biomineralization by the bacterial protein complex Mnx

Lead Institution

University of Washington

Principal Investigator

Bradley Tebo

Project type

Large-Scale EMSL Research

Manganese-oxide mineral formation by bacterial oxidation of dissolved Mn(II) is an important process in aquatic and soil environments and is a key part of the global Mn cycle. The Mn bio-oxides…

Deciphering histone codes underlying diurnal and cell cycle state transitions in Chlamydomonas

Lead Institution

Donald Danforth Plant Science Center

Principal Investigator

James Umen

Project type

Exploratory Research

The long-term goal of this project is to understand the dynamic genetic programming which underlies cell growth and proliferation in green algae. Proliferating microalgae in a diurnal environment are…

Development and Implementation of Online Separation Techniques Coupled to Native Mass Spectrometry for Characterization of Endogenous Proteins Extracted from Biological Matrices

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Recent developments of state-of-the-art mass spectrometry (MS) instrumentation at EMSL, including the 21T Fourier Transform Ion Cyclotron Resonance (FTICR) MS, have enabled high resolution and high…

Development of a functional proteomics workflow for characterization of glycoproteins

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Mowei Zhou

Glycosylation is a diverse family of post-translational modification (PTM) that affects protein structure/function but is not directly encoded in the genome, and represents significant challenge for…

Novel Interactomics Workflow for Protein-Protein and Protein-Lipid Interactions

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Stephanie Thibert

Macromolecular assemblies in cell membrane and organelles are compositionally and structurally complex and their identification and characterization represent a major barrier to understanding…

EMSL Science Overview

Jesse Wilson

Projects

High-throughput native mass spectrometry as experimental validation for in silico drug design

Development of an ECD cell for biomolecular characterization

Manganese biomineralization by the bacterial protein complex Mnx

Deciphering histone codes underlying diurnal and cell cycle state transitions in Chlamydomonas

Development and Implementation of Online Separation Techniques Coupled to Native Mass Spectrometry for Characterization of Endogenous Proteins Extracted from Biological Matrices

Development of a functional proteomics workflow for characterization of glycoproteins

Novel Interactomics Workflow for Protein-Protein and Protein-Lipid Interactions