Lindsey Doyle

Fred Hutchinson Cancer Research Center

Projects

Structural characterization of restriction/modification enzyme BsaXI complex with DNA target for structure-based design of gene-targeting reagents

Lead Institution

Fred Hutchinson Cancer Research Center

Principal Investigator

Betty Shen

Genome engineering and targeted gene modification is a rapidly maturing discipline in which genomes within cell lines, tissues or organisms are manipulated and altered at specified individual loci…

CryoEM structural determinations of Bacterial Restriction-Modification systems

Lead Institution

Fred Hutchinson Cancer Research Center

Principal Investigator

Betty Shen

Nuclease enzymes are of enormous importance for a wide range of biological and biotechnology disciplines that require site-specific DNA manipulations. Bacterial R-M systems consist of a restriction…

Structural characterization of phage defense protein BrxL

Lead Institution

Fred Hutchinson Cancer Research Center

Principal Investigator

Barry Stoddard

Bacterial defense against phage infection is comprised of a large number of biological mechanisms and systems. A thorough understanding of such systems is crucial both for anti-bacterial phage…