Patrick Reardon

Development of NMR Sparse Sampling Methods for Quantitative Metabolic Flux Analysis

Lead Institution

Duke University

Principal Investigator

Brian Coggins

Project type

Large-Scale EMSL Research

Quantitative metabolomics and metabolic flux analysis have emerged in recent years as valuable approaches for the study of carbon cycling in living organisms and in the environment, whether for…

Identifying the mechanism of bacterial extracellular electron transfer via conductive pili proteins

Lead Institution

Yale University

Principal Investigator

Nikhil Malvankar

Project type

Exploratory Research

Microbial metabolism plays an important role in the global cycling of carbon, nutrients, and metals. Recent discovery of microorganisms sharing energy using direct electrical connections might…

The role of phyllosilicate minerals in mediating the temperature sensitivity of enzymatic activity in soil organic matter transformations

Lead Institution

Oregon State University

Principal Investigator

Markus Kleber

Project type

Large-Scale EMSL Research

The underlying idea of this proposal is the assumption that temperature sensitivity in multiple component systems can be expressed by a non-linear combination of two component systems. Our main…

Structural Studies of HIV-1 gp41 MPER and HR-2 Membrane Associated Trimers

Lead Institution

Duke University

Principal Investigator

Leonard Spicer

Project type

Large-Scale EMSL Research

HIV-1 viral-host cell fusion is mediated by the envelope associated gp41 and gp120 protein trimers. The membrane proximal external region (MPER) of gp41 contains the epitopes for the broadly…

Structural Characterization of Bacterial Nanowires

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Karl Mueller

Project type

Large-Scale EMSL Research

Several species of bacteria are capable of reducing insoluble metal oxides as well as other extracellular electron acceptors. These bacteria play a critical role in the cycling of minerals in…

Development of an Integrated EMSL MS and NMR Metabolic Flux Analysis Capability In Support of Systems Biology: Test Application for Biofuels Production

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Isern

An important element in the production of biofuels is the metabolic engineering process required to maximize the production of renewable biofuel molecules from fermentable substrates. Biofuel…

Advancing soil C characterization using HRMS and NMR

Lead Institution

Environmental Molecular Sciences Laboratory

Principal Investigator

Nancy Hess

Soil organic matter consists of diverse and complex mixture of hundreds to thousands biological and metabolic products that are poorly characterized using current techniques. We seek to develop a…

NMR based metabolomics of Apis mellifera, the honeybee, under sub-lethal stress from the neonicotinoid pesticide, Imidacloprid

Lead Institution

Oregon State University

Principal Investigator

Patrick Reardon

Insect pollinators are important components of the environment and agriculture. Recent declines in pollinators, such as the honey bee, could impact global food production and ecosystem stability. …

EMSL Science Overview

Patrick Reardon

Projects

Development of NMR Sparse Sampling Methods for Quantitative Metabolic Flux Analysis

Identifying the mechanism of bacterial extracellular electron transfer via conductive pili proteins

The role of phyllosilicate minerals in mediating the temperature sensitivity of enzymatic activity in soil organic matter transformations

Structural Studies of HIV-1 gp41 MPER and HR-2 Membrane Associated Trimers

Structural Characterization of Bacterial Nanowires

Development of an Integrated EMSL MS and NMR Metabolic Flux Analysis Capability In Support of Systems Biology: Test Application for Biofuels Production

Advancing soil C characterization using HRMS and NMR

NMR based metabolomics of Apis mellifera, the honeybee, under sub-lethal stress from the neonicotinoid pesticide, Imidacloprid