Sneha Couvillion

Investigating the Role of Drought-Impacted Root Exudates on Rhizosphere Microbial Metabolism and Viral Dynamics for Improving Tall Wheatgrass Drought Tolerance

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Sneha Couvillion

Project type

Large-Scale EMSL Research

The rhizosphere is the center of interactions between roots, soil, and microbes, where chemical exchanges are key drivers of microbial composition, plant productivity, and resilience to abiotic…

Linking multi-organism-environment interactions across lab and field scales to estimate viral contributions to soil C cycling

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Amy Zimmerman

Project type

FICUS Research

Interactions between soil viruses and their microbial hosts can significantly impact microbial population dynamics, metabolism, and evolution, with cascading effects on soil carbon (C)…

Metabolomics analysis of carbon storing microbial communities

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan McClure

We have completed the development of two naturally evolved communities of soil bacteria that can produce high levels of CaCO3. As CaCO3 production is one way in which carbon storage in the soil can…

Heracles: In Silico Predictive Tools for Opioid Crisis Intervention

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Katherine Schultz

This project aims to develop software tools to identify and prioritize known and potentially emerging pharmaceutical-based agents and new psychoactive substances (NPS, e.g., variants of illicit drugs…

Mammary and milk microbiomes and metabolomes - Understanding early variation and impacts on risk for mammary inflammation and mastitis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Exclusive breastfeeding for at least 4 mo is considered the optimal form of nutrition for most newborns, yet many women experience substantial roadblocks in meeting this goal. One of the most common…

Prediction of response of microbial interaction networks cycling carbon to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan McClure

Harnessing the soil microbiome presents transformational opportunities to optimize the economic and environmental value of our nation’s soil microbiomes, including maintaining soil organic matter…

Contribution of the endogenous circadian clock to diurnal rhythms in the microbiome of the gut and mouth in humans

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Sneha Couvillion

The gut microbiome, the community of commensal, symbiotic, and pathogenic microorganisms in our intestines, are integral for the host’s digestion and metabolic function, and resistance to infection…

Metabolic profiling to dissect the role of HLA-B27 in axial spondyloarthritis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ernesto Nakayasu

Axial spondyloarthritis (axSpA) and other spondyloarthropathies (SpAs) affect approximately 3 million Americans and are some of the most prevalent rheumatic diseases 18. AxSpA includes ankylosing…

Mechanisms of the protective effects of dietary omega-3 fatty acids and carotenoids in age-related macular degeneration

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ernesto Nakayasu

The goal of the proposed project is to deepen our understanding of the lipid pathways involved in age-related macular degeneration (AMD) and the protective effects of dietary omega-3 fatty acids (…

Comprehensive comparison of taxonomic composition and metabolic functioning in oxygenic and anoxygenic microbial mats of Yellowstone hot springs

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This proposal focuses on comparing a rare, but evolutionarily important, modern laminated microbial mat formed exclusively by anoxygenic photosynthetic microorganisms and a well-studied mat formed by…

Stable Isotope Probing Informatics Infrastructure for High Throughput Analyses

Lead Institution

Michigan State University

Principal Investigator

James Moran

The ability to identify, quantify and trace organic compounds through a soil ecosystem is essential for characterization of the molecular mechanisms that regulate biomolecule interactions with soil…

METABOLOMICS ANALYSES OF THE MICROBIOME FOR DEFENSE HEALTH

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

PNNL will perform both targeted and untargeted metabolomics analyses of human fecal samples to assess the role of diet and dietary components on host health.

MKP1 regulates plant metabolite signals that induce bacterial virulence: How and where are these signals controlled?

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

PNNL will perform both untargeted and targeted metabolomics analyses of plant exudate and tissue samples to assess mechanisms of altered resistance in MKP1

Comprehensive comparison of taxonomic composition and metabolic functioning in oxygenic and anoxygenic microbial mats of Yellowstone hot springs

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This proposal focuses on comparing a rare, but evolutionarily important, modern laminated microbial mat formed exclusively by anoxygenic photosynthetic microorganisms and a well-studied mat formed by…

Mammary and milk microbiomes and metabolomes - Understanding early variation and impacts on risk for mammary inflammation and mastitis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

Exclusive breastfeeding for at least 4 mo is considered the optimal form of nutrition for most newborns, yet many women experience substantial roadblocks in meeting this goal. One of the most common…

Does the brain differentially alter metabolic and immunologic programs in the bone marrow in a pathogen-specific manner?

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Sneha Couvillion

: A wide variety of pathogens present an acute survival challenge in humans, and our ability to rapidly and precisely respond to these pathogens is critical to our survival as a species. Although…

Do Your Gut Microbes Make You Moody?

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Sneha Couvillion

Attention-Deficit/Hyperactivity Disorder (ADHD) is a chronic neurodevelopmental disorder affecting 5- 9% of children and substantial numbers of adults. Co-occurring irritability or mood dysregulation…

Disentangling the role of microbial interactions and spatial structure in soil organic matter decomposition

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan McClure

Project type

Exploratory Research

The study of the soil microbiome is critical to understanding the ecological functions carried out by soil microorganisms, particularly with respect to the cycling of carbon (C) and nitrogen (N)…

Proteomics analysis of ovulation in primates

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Sneha Couvillion

To perform a parallel genomic (RNAseq, University of Oregon) and proteomic (PNNL) assessment of the ovarian follicle in the period prior to and at different time points after an ovulatory stimulus (i…

Prediction of response of microbial interaction networks cycling carbon to changing moisture conditions

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Ryan McClure

Harnessing the soil microbiome presents transformational opportunities to optimize the economic and environmental value of our nation’s soil microbiomes, including maintaining soil organic matter…

Interpretable Machine Learning for Multivariate Omics Data Analysis

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Lisa Bramer

Often, the ultimate motivation for the generation of omics data for an experiment is a better understanding of an observed phenotype of a biological system and the effect of perturbations on the…

Deciphering Microbial Communication Through Metabolites

Lead Institution

Pacific Northwest National Laboratory

Principal Investigator

Thomas Metz

This project will establish a Metabolomics Center at PNNL that will develop the advanced measurement capabilities necessary to define the metabolic processes of microbiomes and determine how these…

EMSL Science Overview

Sneha Couvillion

Projects

Investigating the Role of Drought-Impacted Root Exudates on Rhizosphere Microbial Metabolism and Viral Dynamics for Improving Tall Wheatgrass Drought Tolerance

Linking multi-organism-environment interactions across lab and field scales to estimate viral contributions to soil C cycling

Metabolomics analysis of carbon storing microbial communities

Heracles: In Silico Predictive Tools for Opioid Crisis Intervention

Mammary and milk microbiomes and metabolomes - Understanding early variation and impacts on risk for mammary inflammation and mastitis

Prediction of response of microbial interaction networks cycling carbon to changing moisture conditions

Contribution of the endogenous circadian clock to diurnal rhythms in the microbiome of the gut and mouth in humans

Metabolic profiling to dissect the role of HLA-B27 in axial spondyloarthritis

Mechanisms of the protective effects of dietary omega-3 fatty acids and carotenoids in age-related macular degeneration

Comprehensive comparison of taxonomic composition and metabolic functioning in oxygenic and anoxygenic microbial mats of Yellowstone hot springs

Stable Isotope Probing Informatics Infrastructure for High Throughput Analyses

METABOLOMICS ANALYSES OF THE MICROBIOME FOR DEFENSE HEALTH

MKP1 regulates plant metabolite signals that induce bacterial virulence: How and where are these signals controlled?

Comprehensive comparison of taxonomic composition and metabolic functioning in oxygenic and anoxygenic microbial mats of Yellowstone hot springs

Mammary and milk microbiomes and metabolomes - Understanding early variation and impacts on risk for mammary inflammation and mastitis

Does the brain differentially alter metabolic and immunologic programs in the bone marrow in a pathogen-specific manner?

Do Your Gut Microbes Make You Moody?

Disentangling the role of microbial interactions and spatial structure in soil organic matter decomposition

Proteomics analysis of ovulation in primates

Prediction of response of microbial interaction networks cycling carbon to changing moisture conditions

Interpretable Machine Learning for Multivariate Omics Data Analysis

Deciphering Microbial Communication Through Metabolites