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Characterization of neuropeptides located in neurosecretory tissue of Cancer borealis and Cancer productus.


EMSL Project ID
11595

Abstract

The focal point of my current research is increasing the understanding of peptide signaling in the nervous system through the characterization of neuropeptides located in neurosecretory tissue of Cancer borealis and Cancer productus. Crabs are chosen for model systems in this work for their simplified nervous system yet employing a large diversity of neuropeptides. The characterization is accomplished in two ways; first using matrix assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI FTMS) to obtain tissue specific peptide profiles directly from tissue, secondly, to screen HPLC separations of tissue homogenates pooled from multiple animals to locate and sequence abundant peptides. Also, the peptides released in the circulatory fluid, or hemolymph, are studied to discover changes brought about by changes in physiological conditions such as feeding and day and night cycles.

Project Details

Project type
Exploratory Research
Start Date
2004-10-28
End Date
2005-10-06
Status
Closed

Team

Principal Investigator

Kimberly Kutz
Institution
University of Wisconsin, Madison