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Fluorescent labeling of proteins based on known crystal structures.


EMSL Project ID
13295

Abstract

Protein interactions within living cells are poorly understood, particularly the spatial and temporal associations of ubiqutious proteins like calmodulin (CaM). Here, we propose to utilize computer modeling of known protein crystal structures to determine appropriate labeling sites on Calmodulin and its interacting proteins for thier labeling with small fluorescent probes. This work will identify accessible residues for labeling with FRET compatible probes towards Calmodulin and its interacting proteins.

Project Details

Project type
Exploratory Research
Start Date
2005-02-23
End Date
2006-04-10
Status
Closed

Team

Principal Investigator

Luis Rodriguez
Institution
Pacific Northwest National Laboratory