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Develop a Procedure for the Determination of the Calibration Parameters on the Host Side and Integrate Them into the Hardware to Facilitate Real Time Multiband Analysis


EMSL Project ID
14890

Abstract

Ongoing research at Pacific Northwest National Laboratories aims at
elucidating the complexities behind intercellular communication using the
confocal microscope. The additional Z-plane observation capability of con-
focal microscope provides advantage over standard two-dimensional surface
observations.

The hardware for coregistration of multiband confocal images using cal-
ibration parameters have been developed through research at Utah State
University in collaboration with PNNL. During the SRI period, research
shall be done in developing a procedure for the determination of the cali-
bration parameters on the host side and integrating them into the hardware
to facilitate real time multiband analysis.

This would facilitate further research such as Real-Time Fluorescent Res-
onance Energy Transfer (FRET) Analysis and Real-Time Blob Analysis,
which aid in real-time observation of cell signaling events.Determined by
timing constraints, further work shall be done in hardware implementation
of a working blob analysis algorithm that is currently being developed.

Project Details

Project type
Exploratory Research
Start Date
2005-06-20
End Date
2006-03-27
Status
Closed

Team

Principal Investigator

Marianne Sowa
Institution
NASA Ames Research Center

Team Members

Kenneth Perrine
Institution
Pacific Northwest National Laboratory