Norovirus TEM proposal, Straub
EMSL Project ID
16297
Abstract
Human noroviruses are thought to be the leading cause of non-bacterial gastroenteritis throughout the world. Recent data suggests that the viruse is undergoing mutation leading to increased virulence and causing death in some victims. Efforts to study this virus have been hampered by a lack of a suitable in vitro cell culture system. Our goal is to develop an in vitro cell cultivation system using low-shear microgravity culture systems. Successful cultivation will be measured in several different ways including gross histopathological examination of cell cultures, fluorescent in-situ hybridization, real-time PCR, and electron microscopy. For the purposes of this proposal, we would be seeking access for the electron microscopy facilities at EMSL. Collaborations with EMSL to date suggests that our work in cultivating these viruses has been successful. External peer reviewers have suggested that this work be submitted to Nature because of the unique methods and significant discoveries.
Project Details
Project type
Exploratory Research
Start Date
2005-09-09
End Date
2006-10-04
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Straub TM, Bartholomew RA, Valdez CO, Valentine NB, Dohnalkova A, Ozanich RM, Bruckner-Lea CJ, Call DR. 2010. Human norovirus infection of Caco-2 cells grown as a three dimensional tissue structure. Journal of Water and Health. in press. doi: 10.2166/wh.2010.106
Straub TM, Honer zu Bentrup K, Orosz-Coghlan P, Dohnalkova A, Mayer BK, Bartholomew RA, Valdez CO, Bruckner-Lea CJ, Gerba CP, Abbaszadegan M, Nickerson CA. 2007. In vitro cell culture infectivity assay for human noroviruses. Emerg Infect Dis. 13(3) 396-403.
Straub TM, RA Bartholomew, CO Valdez, NB Valentine, A Dohnalkova, RM Ozanich, CJ Bruckner-Lea, and DR Call. 2011. "Human Norovirus Infection of Caco-2 Cells Grown as a Three-Dimensional Tissue Structure." Journal of Water and Health 9(2):225-240.