Characterization of Post-Translational Modification of the A-Subunit from Heat-Labile Enterotoxin using FTICR Mass Spectrometry
EMSL Project ID
2065
Abstract
Project description: The goal is to use the high mass measurement accuracy provided by FTICR MS to characterize posttranslational modifications of a 26 kDa protein. This protein is the A-subunit of heat-labile enterotoxin, an AB5 toxin from E.coli, associated with cholera-like diarrhea. The A-subunit is an ADP-ribosyl transferase which is capable of self-modification on arginine residues, upon addition of NAD. We are trying to determine how many residues are modified and locate the sites of modification.
Project Details
Project type
Exploratory Research
Start Date
2000-09-20
End Date
2000-11-17
Status
Closed
Released Data Link
Team
Principal Investigator