Sequence-specificities of Hydrogen-Bonded Duplexes
EMSL Project ID
2082
Abstract
This project involves the further characterization of a new class of sequence-specific, H-bonded duplexes we described lately (JACS 1999, v.121, 5607-08; 2000, v. 122, 2635-2644). These H-bonded duplexes may be eventually developed into a whole set of programmable molecular recognition units. The next step includes quantifying the sequence-specificity of these duplexes by introducing mutant strands with mismatched H-bonding sites. Mass spect. method such as those available from EMSL should be powerful and efficient in addressing the specificity question. By detecting the mass of a duplex a in the presence or abasence of other potentially interfering (or mutant) duplexes and/or single strands, the sequence-specificity of that duplex can be quantitatively established. The instrument suitable for the proposed experiments is the TSQ 7000 triple quadrupole mass spectrometer. A total of approximately 5 days are needed.
Project Details
Project type
Exploratory Research
Start Date
2000-11-03
End Date
2002-10-17
Status
Closed
Released Data Link
Team
Principal Investigator