Defining the in vitro phosporylation osites of DNA protein kinase (catalytic subunit)
EMSL Project ID
2148
Abstract
We are in the process of identifying all the in vitro phosphorylation sites on DNA-PKcs.? Because the protein is so large, we were never able to identify the sites by the classical method (isolation of phosphopeptide by HPLC for sequencing by Edmond chemistry).? We will send you in vitro phosphorylated DNA-PKcs for identification of the phosphorylation sites(20 ug) of phosphorylated DNA-PKcs for MS.? Once we have identified all the in vitro sites, we can then confirm wheather they are phosphorylated in vivo by 2-D phosphopepetide analysis.? Eventually, we will send you IPed DNA-PKcs for identification of in vivo sites as well.
Project Details
Project type
Exploratory Research
Start Date
2002-02-28
End Date
2002-02-28
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members