RD NMR Spectroscopy: Rapid Assignment of Proteins for Structural Gennomics
EMSL Project ID
2174
Abstract
NMR structure determinations of proteins rely on nearly complete assignment of chemical shifts, which are routinely obtained using multidimensional 13C, 15N, 1H-triple resonance (TR) NMR experiments. A particular challenge for structural genomics is the development of TR NMR techniques that allows matching of instrument time investments to the minimum time dictated by the NMR signal-to-noise (s/n). In particular, lower bounds for measurement times of three- and four-dimensional NMR experiments are often determined by the targeted digital resolution in the indirect dimensions rather than s/n requirements.
Project Details
Project type
Capability Research
Start Date
2001-07-25
End Date
2001-09-18
Status
Closed
Released Data Link
Team
Principal Investigator