Ultrasensitive mass-spectrometry detection of botulinum toxin A proteolytic activity
EMSL Project ID
2196
Abstract
The major objective is to confirm translocation of botulinum toxin A enzymatic unit through the lipid bilayer using the ultrasensitive assay for its proteolytic activity. Synthetic fragment of SNAP-25 (17 aminoacid residues), will be used as the protease substrate and will be available for the calibration of the instrument. The limiting factor for the assay is expected to be the very low enzymatic subunit concentration in the solution. This increases the demand for high sensitivity of the cleavage products detection and justifies the need for advanced FTICR mass spectrometry.
Project Details
Project type
Exploratory Research
Start Date
2001-10-16
End Date
2002-09-24
Status
Closed
Released Data Link
Team
Principal Investigator