Determination of the proteome of drosophila lipid droplets
EMSL Project ID
2459
Abstract
My research goal is to understand the way molecular motors such as kinesin and dynein move cargos in vivo. To investigate this, I have developed a model system, the bi-directional transport of lipid droplets along microtubules, in early Drosophila embryos. I am a biophysicst by training, and have developed biophysical tools to quantify the motion of individual lipid droplets. In order to better understand this process, I would like to collaborate with scientists at Pacific Northwest national laboratory, to determine the proteome of the lipid droplets. In my lab we would purify the proteins bound to the droplets, and then their identity would be determined using PNL's mass-spectroscopy equipment. Overall, the goal is FUNCTIONAL PROTEOMICS--purify the droplets, determine the proteins bound to them, and then use my biophysical functional assays combined with the power of drosophila genetics, to further understand what the identified proteins do.
Project Details
Project type
Exploratory Research
Start Date
2002-04-15
End Date
2005-04-17
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Bruns EA, VM Perraud, J Greaves, and BJ Finlayson-Pitts. 2010. "Atmospheric Solids Analysis Probe Mass Spectrometry: A New Approach for Airborne Particle Analysis." Analytical Chemistry 82(14):5922–5927. doi:10.1021/ac101028j
Bruns E, V Perraud, A Zelenyuk, MJ Ezell, SN Johnson, Y Yu, D Imre, BJ Finlayson-Pitts, and ML Alexander. 2010. "Comparison of FTIR and Particle Mass Spectrometry for the Measurement of Paticulate Organic Nitrates." Environmental Science & Technology 44(3):1056-1061. doi:10.1021/es9029864
Current Biology 16, 1783-1795, September 19, 2006
Ezell MJ, SN Johnson, Y Yu, V Perraud, E Bruns, ML Alexander, A Zelenyuk, D Dabdub, and BJ Finlayson-Pitts. 2010. "A New Aerosol Flow System for Photochemical and Thermal Studies of Tropospheric Aerosols." Aerosol Science and Technology 44(5):329-338. doi:10.1080/02786821003639700
Kamboures MA, JD Raff, Y Miller, LF Phillips, BJ Finlayson-Pitts, and RB Gerber. 2008. "Complexes of HNO? and NO?¯ with NO? and N?O?, and their potential role in atmospheric HONO formation." Physical Chemistry Chemical Physics. PCCP 10:6019–6032. doi:10.1039/b805330h
Perraud VM, EA Bruns, MJ Ezell, SN Johnson, J Greaves, and BJ Finlayson-Pitts. 2010. "Identification of Organic Nitrates in the NO3 Radical Initiated Oxidation of r-Pinene by Atmospheric Pressure Chemical Ionization Mass Spectrometry." Environmental Science & Technology 44(15):5887–5893. doi:10.1021/es1005658
Perraud VM, EA Bruns, MJ Ezell, SN Johnson, Y Yu, ML Alexander, A Zelenyuk, D Imre, WL Chang, D Dabdub, JF Pankow, and BJ Finlayson-Pitts. 2012. "Nonequilibrium Atmospheric Secondary Organic Aerosol Formation and Growth." Proceedings of the National Academy of Sciences of the United States of America 109(8):2836-2841. doi:10.1073/pnas.1119909109
Yu Y, MJ Ezell, A Zelenyuk, DG Imre, ML Alexander, JV Ortega, B D'Anna, CW Harmon, S Johnson, and BJ Finlayson-Pitts. 2008. "Photooxidation of Alpha-Pinene at High Relative Humidity in the Presence of Increasing Concentrations of NOx." Atmospheric Environment 42(20):5044-5060. doi:10.1016/j.atmosenv.2008.02.026
Yu Y, MJ Ezell, A Zelenyuk, DG Imre, ML Alexander, JV Ortega, JL Thomas, K Gogna, DJ Tobias, B D'Anna, CW Harmon, S Johnson, and BJ Finlayson-Pitts. 2008. "Nitrate Ion Photochemistry at Interfaces: A New Mechanism for Oxidation of alpha-Pinene." Physical Chemistry Chemical Physics. PCCP 10(21):3063-3071. doi:10.1039/b719495a
Yu Y, ML Alexander, V Perraud, E Bruns, S Johnson, M Ezell, and BJ Finlayson-Pitts. 2009. "Contamination from electrically conductive silicone tubing during aerosol chemical analysis." Atmospheric Environment 43(17):2836-2839.
Zelenyuk A, J Yang, and DG Imre. 2009. "Comparison Between Mass Spectra of Individual Organic Particles Generated by UV Laser Ablation and in the IR/UV Two-Step Mode." International Journal of Mass Spectrometry 282(1-2):6-12.
Zelenyuk A, J Yang, C Song, RA Zaveri, and DG Imre. 2008. "A New Real-Time Method for Determining Particles Sphericity and Density: Application to Secondary Organic Aerosol Formed by Ozonolysis of alpha-Pinene." Environmental Science & Technology 42(21):8033-8038. doi:10.1021/es8013562
Zelenyuk A, MJ Ezell, V Perraud, SN Johnson, E Bruns, Y Yu, D Imre, ML Alexander, and BJ Finlayson-Pitts. 2010. "Characterization of Organic Coatings on Hygroscopic Salt Particles and their Atmospheric Impacts." Atmospheric Environment 44(9):1209-1218. doi:10.1016/j.atmosenv.2009.11.047
Zhao R, SJ Ding, Y Shen, DG Camp, II, EA Livesay, HR Udseth, and RD Smith. 2009. "Automated metal-free multiple-column nanoLC for improved phosphopeptide analysis sensitivity and throughput." Journal of Chromatography B 877(8-9):663-670.