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Identification of heat shock proteins in the radioresistant bacterium Deinococcus radiodurans


EMSL Project ID
2547

Abstract

This project aims (1) to identify the proteins present in the proteome of Deinococcus radiodurans R1 under heat shock conditions and (2) to identify proteins under the control of heat shock regulators in Deinococcus radiodurans. To achieve this aim, the proteome of heat shocked and exponentially growing Deinococcus radiodurans will be compared using the high-throughput mass spectrometry proteomic techniques developed by Richard D. Smith. The proteins that are present in the wild type strain will then be compared by whole-cell mass spec to heat shocked mutant strains that lack important heat shock regulators. Proteins that are absent in the mutants under heat shock are expected to represent proteins under the control of the heat shock regulators. These studies will enable an in-depth, proteome-wide characterization of heat shock in Deinococcus radiodurans.

Project Details

Project type
Exploratory Research
Start Date
2002-06-17
End Date
2004-06-28
Status
Closed

Team

Principal Investigator

Amy Schmid
Institution
Duke University