Automated Image Analysis of Low Frequency Radiation Induced Genomic Instability in Human Colon Carcinoma Cells
EMSL Project ID
25600
Abstract
This proposal is a continuation of research under EMSL User Proposal #21990. Ionizing radiation induced bystander effects are those effects occurring in cells that were not traversed by radiation but were induced by signals from irradiated cells. Bystander effects have been described in numerous in vitro cell culture systems and there is also evidence that they can occur in vivo in complex model systems and animals. The fact that many of the effects associated with cellular exposure to radiation can manifest in non-irradiated bystander cells has implications for any individual who may be exposed to ionizing radiation because they suggest that the target for radiation responses might be greater than the volume actually irradiated. Investigating potentially rare events occurring in cells surviving exposure to ionizing radiation, demands an assay that can rapidly and reliable screen very large numbers of cells. Using a GFP reporter construct developed in the Morgan laboratory, we will develop a high throughput method of automated image analysis to provide the necessary statistical power for the investigation of rare radiation induced instability.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2007-07-18
End Date
2008-08-13
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members