Skip to main content

51V Solid-State MAS NMR studies of vanadium haloperoxidases


EMSL Project ID
26699

Abstract

Solid-state 51V NMR spectra will be acquired at 21.1T of vanadium haloperoxidases, chloro- and bromoperoxidase. These enzymes are present in marine microalgae, terrestrial fungi, and in some lichens and are thought to be involved in the biosynthesis of halogenated natural products. The spectra acquired at 21.1T will compliment those acquired at 14.1T in our lab and allow us to determine both the electric field gradient, EFG, and chemical shift, CS, tensors. By acquiring spectra of both wild type and mutant proteins we can hope to gain an understanding of the electronic structure of the vanadium center as well as obtain structural information about the active sites. The use of the 21.1T NMR spectrometer is necessary for this characterization as it will allow us to acquire spinning side band patterns for the 51V satellite transitions which are difficult to do at lower magnetic field strengths, based on our preliminary results. The added signal-to-noise achieved by using the higher field strength will also be invaluable in determining the NMR parameters.

Project Details

Project type
Capability Research
Start Date
2007-10-01
End Date
2008-10-10
Status
Closed

Team

Principal Investigator

Tatyana Polenova
Institution
University of Delaware

Team Members

Stephanie Bolte
Institution
University of Delaware

Kristopher Ooms
Institution
The King's University College