Biotemplated nanoparticles for imaging and drug delivery
EMSL Project ID
30493
Abstract
The objective of this project is to develop new approaches to prepare encoded bimetallic nanoparticles in protein cages for biosensing and imaging. The unique optical and electrochemical signals of encoded bimetallic alloys, which depend on composition ratios of metal alloys, can be used for multiplexed detection and as fingerprints of a specific protein biomarker and reduce false positive alarm. The specific aims of the project are: 1) understand the effect of solution chemistry and protein cage chemistry on the diffusion and reduction of different metal ions, and 2) facilitate a new multiplex sensing platform based on electrochemical and optical techniques.
Project Details
Project type
Large-Scale EMSL Research
Start Date
2008-09-01
End Date
2009-09-30
Status
Closed
Released Data Link
Team
Principal Investigator
Team Members
Related Publications
Chen A, Y Bao, X Ge, Y Shin, D Du, and Y Lin. 2012. "Magnetic Particle-Based Immunoassay of Phosphorylated p53 Using Protein-Cage Templated Lead Phosphate and Carbon Nanospheres for Signal Amplification." RSC Advances 2(29):11029-11034. doi:10.1039/C2RA20994B
Du D, J Wang, D Lu, A Dohnalkova, and Y Lin. 2011. "Multiplexed Electrochemical Immunoassay of Phosphorylated Proteins Based on Enzyme-Functionalized Gold Nanorod Labels and Electric Field-Driven Acceleration." Analytical Chemistry 83(17):6580-6585. doi:10.1021/ac2009977
Du D, J Wang, L Wang, D Lu, and Y Lin. 2012. "Integrated Lateral Flow Test Strip with Electrochemical Sensor for Quantification of Phosphorylated Cholinesterase: Biomarker of Exposure to Organophosphorus Agents." Analytical Chemistry 84(3):1380-1385. doi:10.1021/ac202391w
Du D, Z Zou, Y Shin, J Wang, H Wu, MH Engelhard, J Liu, IA Aksay, and Y Lin. 2010. "Sensitive Immunosensor for Cancer Biomarker Based on Dual Signal Amplification Strategy of Graphene Sheets and Multi-Enzyme Functionalized Carbon Nanospheres ." Analytical Chemistry 82(7):2989-2995. doi:10.1021/ac100036p
Kang X, J Wang, H Wu, J Liu, IA Aksay, and Y Lin. 2010. "A graphene-based electrochemical sensor for sensitive detection of paracetamol ." Talanta 81(3):754-759.
Lu D, J Wang, L Wang, D Du, C Timchalk, RC Barry, and Y Lin. 2011. "A novel nanoparticle-based disposable electrochemical immunosensor for diagnosis of exposure to toxic organophosphorus agents." Advanced Functional Materials 21(22):4371-4378. doi:10.1002/adfm.201100616
Ma Ham A, HJ Wu, J Wang, X Kang, Y Zhang, and Y Lin. 2011. "Apoferritin-based nanomedicine platform for drug delivery: equilibrium binding study of daunomycin with DNA." Journal of Materials Chemistry 21(24):8700-8708 . doi:10.1039/C0JM04321D
Ma Ham A, Z Tang, H Wu, J Wang, and Y Lin. 2009. "Protein-Based Nanomedicine Platforms for Drug Delivery." Small 5(15):1706-1721. doi:10.1002/smll.200801602
Shao Y, J Wang, MH Engelhard, CM Wang, and Y Lin. 2010. "Facile and controllable electrochemical reduction of graphene oxide and its applications." Journal of Materials Chemistry 20(4):743-748.
Tang Z, HJ Wu, Y Zhang, Z Li, and Y Lin. 2011. "The Enzyme-mimic Activity of Ferric Nano-Core Residing in Ferritin and Its Biosensing Applications." Analytical Chemistry 83(22):8611–8616. doi:10.1021/ac202049q
Wang Y, Z Li, J Wang, J Li, and Y Lin. 2011. "Graphene and Graphen Oxide: Biofunctionalization and Applications in Biotechnology." Trends in Biotechnology 29(5):205-212. doi:10.1016/j.tibtech.2011.01.008
Wu H, J Wang, X Kang, CM Wang, D Wang, J Liu, IA Aksay, and Y Lin. 2009. "Glucose Biosensor Based on Immobilization of Glucose Oxidase in Platinum Nanoparticles/Graphene/Chitosan Nanocomposite Film." Talanta 80(1):403-406. doi:10.1016/j.talanta.2009.06.054
Zhang S, Y Shao, G Yin, and Y Lin. 2010. "Carbon nanotubes decorated with Pt nanoparticles via electrostatic self-assembly: a highly active oxygen reduction electrocatalyst ." Journal of Materials Chemistry 20(15):2826-2830. doi:10.1039/b919494k
Zhang S, Y Shao, G Yin, and Y Lin. 2010. "Electrostatic Self-Assembly of Pt-around-Au Nanocomposite with High Activity towards Formic Acid Oxidation." Angewandte Chemie International Edition 49(12):2211-2214. doi:10.1002/anie.200906987
Zhang Y, Z Tang, J Wang, HJ Wu, CT Lin, and Y Lin. 2011. "Apoferritin Nanoparticle: A Novel and Biocompatible Carrier for Enzyme Immobilization with Enhanced Activity and Stability." Journal of Materials Chemistry 21(43):17468-17475. doi:10.1039/c1jm11598g