Molecular dysfunction in alcoholic liver disease.
EMSL Project ID
3671
Abstract
Mice transgenic for the human Cytochrome P450 2E1 (primary metabolizer of alcohols in the liver) were fed alcohol intra-gastrically for a month. Upon examination of the liver microscopically, inclusions (arrows) in liver cells (alcoholic) were detected. These were micro-dissected out from fixed and sectioned tissue (and called "experimental") and the "control" sample is normal cytoplasm from the same liver cells that did not have inclusions. We have succeeded in extracting protein out of the cross-linked material and run them in a gel. The protein is behaving well (silver stained gel figure provided). We would like to collaborate to try to resolve the identity of proteins in the alcoholic liver inclusion bodies. This identification will not only shed light on alcoholic liver disease but also (probably) on liver diseases related to (oxidative) stress and (ROS) related injury.
Project Details
Project type
Exploratory Research
Start Date
2003-08-26
End Date
2004-02-16
Status
Closed
Released Data Link
Team
Principal Investigator