Investigating Molecular Recognition and Biological Function at Interfaces Using Antimicrobial and Biomineralization Peptides
EMSL Project ID
3994
Abstract
My research plans reflect my fundamental interest in the principles underlying the biological function and the mechanisms of action of peptides active at interfaces. Complementary solid state Nuclear Magnetic Resonance (NMR) techniques will be used on site-specific isotopically labeled peptides. Syntheses of these key compounds and the sample preparations will be performed at Pacific Lutheran University (PLU, Tacoma, WA, http://www.plu.edu/), where I am now a faculty, and at a major research university (Department of Chemistry at the UW, University of Washington, Seattle, WA), which hosted me as a postdoctoral scientist. In order to permit progress towards the goals of this proposal, it is hoped that the present request will be successful in providing some time on the EMSL 500 WB NMR spectrometer. I am applying as the ?primary author? but I would like one or two PLU students to be considered access too. In the project outlined here, the mechanisms of action of antimicrobial peptides from mast cells of fish will be studied through the investigation of structure/dynamics/function relationships. These topics will be addressed by a combination of emerging solid state NMR techniques, which I have successfully used myself in my graduate and post-doctoral research. A second project, not described here, pertains to biomineralization peptides involved in protein assemblies and the microstructure of minerals. Understanding molecular recognition at interfaces can provide some important knowledge needed to fight many diseases and design new drugs, including broad-spectrum ones. Beyond this scientific motivation, a paramount goal of this project is also to provide an environment in which undergraduate students can integrate learning and advanced research. Access to the state of the art facility at EMSL would be pivotal to achieving these pursuits, both from research and educational standpoints. Thank you for your consideration.
Project Details
Project type
Capability Research
Start Date
2004-01-07
End Date
2005-01-13
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
A. Certain, T.R. Allen, D. Hoelzer, C. Parish, S. Shutthanandan, S. Kuchibhatla, J. Taylor, Y. Wu, Study of Nanocluster Stability Nanostructured Ferritic Alloys (NFAs) Under High Dose Irradiation, ICFRM15, Charleston, S.C., October 2011
A. Certain, T.R. Allen, S. Kuchibhatla, V. Shutthanandan, D. T. Hoelzer, Study of Nanocluster Stability Nanofeatured ODS Steels Under High Dose Ion Irradiation and Low Dose Neutron Irradiation, TMS 2012, Orlando, FL March 15th, 2012
A. Mairov, B. Hauch, K. Sridharan, T. Allen, J. Zhang, Radiation Stability of Thin Film Interfaces, Materials Science and Technology Conference, Pittsburgh, PA, October 11th, 2012
Certain AG. 2012. Radiation Stability of Nanoclusters in Nanofeatured Oxide Dispersion Strengthened (ODS) Steel . Pacific Northwest National Laboratory, Richland, WA.
Certain AG, SVNT Kuchibhatla, V Shutthanandan, DT Hoelzer, and TR Allen. 2012. "Radiation Stability of Nanoclusters in Nano-structured Oxide Dispersion Strengthened (ODS) Steels." PNNL-SA-89292, Pacific Northwest National Laboratory, Richland, WA.
Certain AG, SVNT Kuchibhatla, V Shutthanandan, DT Hoelzer, and TR Allen. 2013. "Radiation Stability of Nanoclusters in Nano-structured Oxide Dispersion Strengthened (ODS) Steels." Journal of Nuclear Materials 434(1-3):311-321.
Fanizza, M.F., H. Yoon, C. Zhang, M. Oostrom, T.W. Wietsma, N.J. Hess, M.E. Bowden, T.J. Strathmann, K.T. Finneran, C.J. Werth, Pore Scale Evaluation of Uranyl Phosphate Precipitation in a Model Groundwater System, Water Resour. Res., DOI: 10.1002/wrcr.20088, Feb, 2013.
Kim B, Z Li, BD Kay, Z Dohnalek, and YK Kim. 2012. "Unexpected Nondissociative Binding of N2O on Oxygen Vacancies on a Rutile TiO2(110)-1×1 ." Journal of Physical Chemistry C 116(1):1145-1150. doi:10.1021/jp210636j
Kim B, Z Li, BD Kay, Z Dohnalek, and YK Kim. 2014. "Low-Temperature Desorption of N2O from NO on Rutile TiO2(110)-1x1." Journal of Physical Chemistry C 118(18):9544-9550. doi:10.1021/jp501179y
Li Z, BD Kay, and Z Dohnalek. 2013. "Dehydration and Dehydrogenation of Ethylene Glycol on Rutile TiO2(110)." Physical Chemistry Chemical Physics. PCCP 15(29):12180-12186. doi:10.1039/c3cp50687h
Li Z, Z Zhang, BD Kay, and Z Dohnalek. 2011. "Polymerization of Formaldehyde and Acetaldehyde on Ordered (WO3)3 Films on Pt(111)." Journal of Physical Chemistry C 115(19):9692-9700. doi:10.1021/jp202169x
May RA, RS Smith, and BD Kay. 2013. "The Release of Trapped Gases from Amorphous Solid Water Films: II. “Bottom-Up” Induced Desorption Pathways." Journal of Chemical Physics 138(10):Article No. 104502. doi:10.1063/1.4793312
May RA, RS Smith, and BD Kay. 2013. "The Release of Trapped Gases from Amorphous Solid Water Films: I. “Top-Down” Crystallization-Induced Crack Propagation Probed using the Molecular Volcano." Journal of Chemical Physics 138(10):Article No. 104501. doi:10.1063/1.4793311
Mckenna KP. 2013. "Electronic and Chemical Properties of a Surface Terminated Screw Dislocation in MgO." Journal of the American Chemical Society 135(50):18859-18865. doi:10.1021/ja408342z
Mckenna KP, AL Shluger, V Iglesias, M Porti, M Lanza, and G Bersuker. 2011. "Grain boundary mediated leakage current in polycrystalline HfO2 films ." Microelectronic Engineering 88(7):1272-1275. doi:doi:10.1016/j.mee.2011.03.024
Mckenna KP, and AL Shluger. 2011. "Electron and Hole Trapping in Polycrystalline Metal Oxide Materials." Royal Society of London. Proceedings A. Mathematical, Physical and Engineering Sciences 467(2131):2043-2053. doi:10.1098/rspa.2010.0518
Mckenna KP, and AL Shluger. 2013. "Structure and Electronic Properties of Polycrystalline Dielectrics." ECS Transactions 54(1):243-253. doi:10.1149/05401.0243ecst
Mckenna KP, D Koller, A Sternig, N Siedl, N Govind, PV Sushko, and OE Diwald. 2011. "Optical Properties of Nanocrystal Interfaces in Compressed MgO Nanopowders." ACS Nano 5(4):3003–3009. doi:10.1021/nn200062d
oral presentation:A. Mairov, B. Hauch, K. Sridharan, T. Allen "Stability of Fe-12%Cr Thin Films Interfaces Under Heavy Ion Irradiation", Materials Science and Technology Annual Conference, Pittsburgh, PA, October 12-16, 2014.
poster: A. Mairov, B. Hauch, K. Sridharan, T. Allen, J. Zhang "Stability of a Titanium- and Yttrium- Fe-12%Cr Interface Under Heavy Ion Irradiation", TMS Conference, San Diego, CA, February 16-20, 2014
Poster - T. Gerczak, Z. Zhu, T. R. Allen, Silver Diffusion in SiC, AVS Pacific Northwest Chapter of the AVS Annual Symposium, Environmental and Molecular Sciences Laboratory, PNNL, Richland, WA September 16th - 17th, 2010
Schaef HT, CF Windisch, Jr, BP McGrail, PF Martin, and KM Rosso. 2011. "Brucite [Mg(OH2)] Carbonation in Wet Supercritical CO2: An in situ High Pressure X-Ray Diffraction Study." Geochimica et Cosmochimica Acta 75(1):7458-7471. doi:10.1016/j.gca.2011.09.029
Schaef HT, QRS Miller, CJ Thompson, JS Loring, ME Bowden, BW Arey, BP McGrail, and KM Rosso. 2013. "Silicate Carbonation in Supercritical CO2 Containing Dissolved H2O: An in situ High Pressure X-Ray Diffraction Study." Abstract submitted to GHGT11, Kyoto, Japan. PNNL-SA-85671. Published in Silicate Carbonation in Supercritical CO2 Containing Dissolved H2O: An in situ High Pressure X-Ray Diffraction and Infrared Spectroscopy Study , vol. 37, pp. 5892-5896. doi:10.1016/j.egypro.2013.06.514
Shluger AL, KP Mckenna, PV Sushko, DM Ramo, and AV Kimmel. 2009. "Modelling of electron and hole trapping in oxides." Modelling and Simulation in Materials Science and Engineering 17(084004):21. doi:10.1088/0965-0393/17/8/084004
Tang M ,Zhang Z ,Ge Q 2016. "A DFT-based Study of Surface Chemistries of Rutile TiO2 and SnO2(110) Toward Formaldehyde and Formic Acid" Catalysis Today 274():103–108. 10.1016/j.cattod.2016.01.057
T. Gerczak, G. Zheng, T. R. Allen, "Investigation on Ag and Cs Diffusion in SiC," The 2nd workshop on HTGR SiC Material Properties, January 20, 2012 Idaho Falls, ID
T. Gerczak, G. Zheng, Z. Zhu, T.R. Allen, "Cesium and Silver Diffusion for TRISO Applications," 2011 American Nuclear Society Summer Meeting, American Nuclear Society, Hollywood, FL , Summary 3897
T. Gerczak, T. R. Allen, N. Kita, Z. Zhu "Insights on Ag and Cs Diffusion in SiC," Materials Science and Technology Conference, Columbus, OH October 18th, 2011
T. Gerczak, T. R. Allen, "Role of Microstucture on Ag and Cs Diffusion in SiC," TMS 2012, Orlando, FL March 15th, 2012
Wang Y, D Mei, J Li, and RJ Rousseau. 2013. "DFT+U Study on the Localized Electronic States and Their Potential Role During H2O Dissociation and CO Oxidation Processes on CeO2(111) Surface." Journal of Physical Chemistry C 117(44):23082-23089. doi:10.1021/jp409953u
Wang Y, Y Yoon, VA Glezakou, J Li, and RJ Rousseau. 2013. "The Role of Reducible Oxide-Metal Cluster Charge Transfer in Catalytic Processes: New Insights on The Catalytic Mechanism of CO Oxidation on Au/TiO2 from Ab Initio Molecular Dynamics." Journal of the American Chemical Society 135(29):10673-10683. doi:10.1021/ja402063v
Windisch CF, Jr, VA Glezakou, PF Martin, BP McGrail, and HT Schaef. 2012. "Raman Spectrum of Supercritical C18O2 and Re-Evaluation of the Fermi Resonance." Physical Chemistry Chemical Physics. PCCP 14(8):2560–2566. doi:10.1039/c1cp22349f
Xia Y, B Zhang, J Ye, Q Ge, and Z Zhang. 2012. "Acetone-Assisted Oxygen Vacancy Diffusion on TiO2(110)." Journal of Physical Chemistry Letters 3(20):2970–2974. doi:10.1021/jz301293y
Ye J, C-j Liu, D Mei, and Q Ge. 2014. "Methanol synthesis from CO2 hydrogenation over a Pd4/In2O3 model catalyst: A combined DFT and kinetic study." Journal of Catalysis 317:44-53. doi:10.1016/j.jcat.2014.06.002