Phenotypic characterization of TCE and PCE degrading Dehalococcoides
EMSL Project ID
4091
Abstract
We presently have in our laboratory several anaerobic mixed cultures, enriched from contaminated aquifer soils, capable of reductively dechlorinating TCE or PCE fully to ethene in the presence of butanol or hydrogen as electron donors. Preliminary PCR analysis indicates abundant Dehalococcoides in these cultures. To date, the vast majority of studies on dehalogenators and Dehalococcoides specifically have been primarily mechanistic, with more recent work focusing on molecular characterization. Virtually no work has been done on visualizing dehalogenators. We wish to use the SEM and TEM capabilities at EMSL to image these microorganisms from our cultures.
Project Details
Project type
Exploratory Research
Start Date
2003-09-02
End Date
2005-10-06
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Rajachidambaram MS, T Varga, L Kovarik, RP Sanghavi, V Shutthanandan, S Thevuthasan, S Han, C Chang, and GS Herman. 2012. "Formation of zinc oxide films using submicron zinc particle dispersions." Journal of Vacuum Science and Technology B--Microelectronics and Nanometer Structures 30(4):Article No. 041805. doi:10.1116/1.4731255