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Making Isoprene from Biomass Material using Bacillus Species


EMSL Project ID
41296

Abstract

Isoprene, also known as 2-methyl-1, 3-butadiene, is a volatile, five-carbon, pure hydrocarbon. Isoprene is derived from biomass through a biochemical process and can be utilized as a fossil fuel alternative and a platform chemical to produce high-value bio-based chemicals. The major aim of this proposal is: using EMSL mass spectrometry capability to identify unknown activators and repressors of the DXP pathway in the B. subtilis strain DSM10 by comparing the proteomic profile between two growth conditions (control and heat). These unknown regulators can be utilized as targets for future genetic modification.

Project Details

Project type
Limited Scope
Start Date
2010-12-01
End Date
2011-01-18
Status
Closed

Team

Principal Investigator

Birgitte Ahring
Institution
Washington State University Tri-Cities

Team Members

Junfeng Xue
Institution
Washington State University Tri-Cities