Mass spectroscopy of a recombinant ribose sensor cassette
EMSL Project ID
44994
Abstract
Follow-up analysis is needed to complete research initiated on EMSL User Facility Proposal 24798 entitled 'Characterization of Application-Relevant Biosilica-Precipitating Peptides of a Diatom'. Research on this topic area is continuing, and there is a need for timely verification of a ribose sensor cassette that is being used to transform diatoms. The results of mass spectrometry of our construct have been inconclusive. We would like to resubmit new protein isolated through an optimized protein purification technique for timely analysis of mass and peptide sequence. These data would provide a strong package not only for our presentations but also, would provide a capstone for the publication we are currently writing. Due to time constraints with the conference and program review quickly approaching, a rapid turnaround would be necessary for the proposed work.
Project Details
Project type
Limited Scope
Start Date
2011-05-16
End Date
2011-07-16
Status
Closed
Released Data Link
Team
Principal Investigator
Related Publications
Marshall KE, EW Robinson, SM Hengel, L Pasa-Tolic, and G Roesijadi. 2012. "FRET Imaging of Diatoms Expressing a Biosilica-Localized Ribose Sensor." PLoS One 7(3):e33771 1-8. doi:10.1371/journal.pone.0033771