Expression profile of biomass-degrading fungi inhabiting the cow rumen
EMSL Project ID
47401
Abstract
Fungi play a significant role in anaerobic degrading of biomass in the cow rumen and fungal enzymes appear to have higher specific activities towards plant cell wall polymers than bacterial biomass-degrading enzymes. To identify and catalogue the biomass-degrading enzymes that are produced by fungi and other eukaryotes that colonize and degrade switchgrass during rumen-incubation, my group teamed up with several researchers from PNNL, the DOE Joint Genome Institute and other institutes to generate an expression profile of eukaryotic rumen microbes. The catalogue of biomass-degrading enzymes that will result from this project will represent a valuable resource for scientists developing more efficient processes for the production of cellulosic biofuels. High-quality non-degraded RNA will be essential for the success of this project. EMSL equipment for RNA extraction and analysis would facilitate the extraction of non-degraded RNA and contribute significantly to the success of this project and therefore I am applying to obtain access to some basic instruments available at EMSL.
Project Details
Start Date
2012-03-26
End Date
2013-03-31
Status
Closed
Released Data Link
Team
Principal Investigator
Co-Investigator(s)
Related Publications
Piao H, LM Markillie, DE Culley, RI Mackie, and M Hess. 2013. "Improved Method for Isolation of Microbial RNA from Biofuel Feedstock for Metatranscriptomics." Advances in Microbiology 3(1):101-107. doi:10.4236/aim.2013.31016