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Hematopoietic Stem and Progenitor Cell Kinetics of Irradiated Minipigs


EMSL Project ID
47771

Abstract

There is a need for a well-characterized animal model to test efficacy and safety of developmental prophylactics and therapeutics for acute radiation exposure in humans. We propose to characterize the kinetic profile of hematopoietic stem and progenitor cells in bone marrow aspirates of irradiated minipigs. Cell-specific antigens will be labeled with fluorescently tagged antibodies, and cells will be quantified using flow cytometry at the Environmental Molecular Science Laboratory (EMSL). The kinetic profile of hematopoietic stem and progenitor cells will provide well-characterized biomarkers of acute radiation syndrome in the minipig.

Project Details

Start Date
2013-01-15
End Date
2013-09-30
Status
Closed

Team

Principal Investigator

Jordan Smith
Institution
Pacific Northwest National Laboratory