Deep RNA Sequencing and Proteomic Analysis of a Hybrid Mouse Diversity Panel
EMSL Project ID
47793
Abstract
This project will chart with high-resolution the genetic pathways that give rise to defined clinica phenotypes. We will utilize the power of the hybrid mouse diversity panel (HMDP), combined with massive-scale RNA sequencing and proteomics, to trace the interconnections from DNA to RNA to protein and provide layered information on the production of medically relevant traits. The HMDP consists of 100 inbred and recombinant inbred strains. The panel has a wide array of meiotic breakpoints and has been densely genotyped with more than 140,000 single nucleotide polymorphisms (SNPs), allowing very fine mapping of quantitative trait loci (QTLs). In addition, the HMDP is genetically stable and renewable and can be assayed for multiple phenotypes, yielding cumulative biological information. We will expand our previous HMDP-based studies of metabolic and behavioral disorders, adding massive-scale RNA sequencing (RNA-Seq) and proteomics to detail genetic control of protein expression. Our approach offers the distinct advantage of mapping loci for known pathways as well as variant RNA and protein species.EMLS mass spectrometry instrumentation provides a unique capability for ultra-deep, accurate and precise quantitation across the ~100 of HMDP mouse strains.
Project Details
Start Date
2013-02-25
End Date
2015-09-30
Status
Closed
Released Data Link
Team
Principal Investigator