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Building the lignin metabolic map for the production of advanced biofuels


EMSL Project ID
48673

Abstract

The overall goal of this project is to develop a metabolic map in four targeted organisms using 13C-labeled versions of the lignin-derived model compounds known to be capable of supporting microbial growth by analyzing a time series of growth using these compounds as the sole carbon source. Time courses of 13C uptake and conversion of model lignin-derived compounds will be measured by metabolomics (HRMAC), proteomics (Orbitrap), and transcriptomics (RNASeq) in four microbes known to be capable of growing on non-labeled versions of the same lignin intermediates to build and compare the lignin metabolic map in each. Maps of lignin degradation metabolism for the four microbes will be built using BNICE framework based on the results. These metabolic maps will be the first step in developing a synthetic biology toolbox for the engineering of organisms capable of producing biofuels from lignin.

Project Details

Project type
Special Science
Start Date
2014-12-23
End Date
2016-09-30
Status
Closed

Team

Principal Investigator

Blake Simmons
Institution
Lawrence Berkeley National Laboratory

Co-Investigator(s)

Kristen DeAngelis
Institution
University of Massachusetts Amherst

Team Members

John Gladden
Institution
Joint BioEnergy Institute

Steven Singer
Institution
Lawrence Berkeley National Laboratory